超声耦合大孔树脂固定床吸附与解吸附黑果腺肋花楸花色苷的传质特性  

Mass Transfer Characteristics during Ultrasound-Assisted Adsorption and Desorption of Aronia melanocarpa Anthocyanins on Fixed Bed Filled with Macroporous Resin

作　　者：戴妍贤 沈娟[1] 龚文进 韩永斌[1,2] 李丹丹[1,2] 陶阳[1,2] DAI Yanxian;SHEN Juan;GONG Wenjin;HAN Yongbin;LI Dandan;TAO Yang(College of Food Science and Technology,Nanjing Agricultural University,Nanjing 210095,China;Whole Grain Food Engineering Research Center,Nanjing Agricultural University,Nanjing 210095,China)

机构地区：[1]南京农业大学食品科技学院,江苏南京210095 [2]南京农业大学全谷物食品工程研究中心,江苏南京210095

出　　处：《食品科学》2023年第21期69-80,共12页Food Science

基　　金：国家自然科学基金面上项目(32072351);江苏省农业科技自主创新项目(CX(22)2026)。

摘　　要：目的:构建超声耦合大孔树脂固定床装置用于黑果腺肋花楸花色苷的分离纯化,探究超声对固定床吸附/解吸附黑果腺肋花楸花色苷传质特性的影响。方法:检测超声耦合固定床纯化过程中总花色苷、可溶性糖、有机酸、单体花色苷及其他多酚的传质规律,并分析超声处理后大孔树脂物理性质变化规律。结果:本研究所用装置可有效除去黑果腺肋花楸花色苷粗提物中的杂质,纯化后黑果腺肋花楸花色苷和多酚纯度分别可达48.8%和78.7%。吸附过程中,超声促进了花色苷的溶出,18.76 W/cm^(2)超声作用于2.5 cm柱床时,花色苷吸附突破时间缩短65.8%。较高极性洗脱液用于固定床解吸附时,超声处理强化了花色苷的解吸附传质,采用25%乙醇溶液洗脱48 min后,18.76 W/cm^(2)超声作用下花色苷解吸附量提高了12.6%。超声作用明显增加了大孔树脂表面的粗糙度,但树脂结构无明显改变。结论:超声耦合固定床处理可明显提高吸附/解吸附过程中花色苷在树脂颗粒间的流动性,但是对树脂颗粒与花色苷间的相互作用影响不大。In this study,an ultrasound-assisted macroporous resin fixed-bed device was constructed for the purification of Aronia melanocarpa anthocyanins.Meanwhile,the effect of ultrasound on the mass transfer characteristics during the adsorption/desorption of anthocyanins on the fixed bed was investigated.The mass transfer patterns of total anthocyanins,total sugars,individual organic acids,individual anthocyaninic and non-anthocyaninic phenolics during fixed-bed purification were determined,and the effect of ultrasound on the physical properties of macroporous resins was analyzed.It was found that this fixed bed device could effectively remove impurities from the crude extract of A.melanocarpa.After purification,the purities of anthocyanin and polyphenol were 48.8%and 78.7%,respectively.During adsorption,ultrasound promoted the release of anthocyanins from the fixed bed.Specifically,in the fixed bed with a height of 2.5 cm,the breakthrough time of anthocyanin adsorption was reduced by 65.8%under sonication at 18.76 W/cm^(2).Moreover,the mass transfer during anthocyanin desorption using a highly polar eluent was enhanced by ultrasound.When 25%aqueous ethanol was used as the desorption solvent,the amount of anthocyanins desorbed from the fixed bed after 48 min of elution was increased by 12.6%by 18.76 W/cm^(2) ultrasound.Besides,ultrasound significantly increased the surface roughness of macroporous resin particles,but did not change the interior structure.In summary,ultrasound-assisted fixed bed treatment significantly improves the flowing of anthocyanins among resin particles during the adsorption/desorption,but has little effect on the interaction between single resin particles and anthocyanins.

关 键 词：黑果腺肋花楸 花色苷 超声 吸附 解吸附 传质 

分 类 号：TS207.3[轻工技术与工程—食品科学] TS255.1[轻工技术与工程—食品科学与工程]