蓝莓果渣多酚-纳米硒的制备、表征及抑菌活性  

Preparation,Characterization and Antibacterial Activity of Blueberry Pomace Polyphenol-Selenium Nanoparticles

作　　者：徐雅琴[1] 钟敬伟 李国强 王欣茹 常松涛 杨昱[1] 王丽波[1] XU Yaqin;ZHONG Jingwei;LI Guoqiang;WANG Xinru;CHANG Songtao;YANG Yu;WANG Libo(College of Arts and Sciences,Northeast Agricultural University,Harbin 150030,China)

机构地区：[1]东北农业大学文理学院,黑龙江哈尔滨150030

出　　处：《食品科学》2023年第22期9-15,共7页Food Science

基　　金：国家自然科学基金面上项目(32172916);东北农业大学大学生创新创业计划项目(SIPT);黑龙江省自然科学基金项目(LH2021C044)。

摘　　要：采用超声辅助双水相法提取蓝莓果渣多酚,经大孔树脂X-5纯化后用于制备蓝莓果渣多酚-纳米硒(total polyphenol-selenium nanoparticles,TP-SeNPs)。通过能量色散X射线光谱、傅里叶变换红外光谱、透射电子显微镜和X射线衍射光谱表征TP-SeNPs结构,并测定其抑菌活性。结果显示:硒盐经还原变为单质形态硒,TP的O—H基团和纳米硒原子之间有着类似氢键的相互作用,TP-SeNPs稳定性良好、呈近似球状分散分布的无定形固体、TP-SeNPs对革兰氏阳性菌的抑制效果优于革兰氏阴性菌,其中对李斯特菌的最低抑菌浓度为0.06 mg/mL。TP-SeNPs可有效抑制李斯特菌生物被膜的形成,破坏李斯特菌细胞壁与细胞膜的通透性与完整性,造成细胞内容物泄漏,导致李斯特菌死亡。本研究可为蓝莓果渣与纳米硒的应用提供新思路,为纳米硒抑菌剂的开发提供理论依据。Total polyphenol-selenium nanoparticles(TP-SeNPs)were prepared with blueberry pomace polyphenols,prepared by ultrasonic assisted aqueous two-phase extraction and purified by using macroporous resin X-5.The structure of TP-SeNPs was characterized by energy dispersive X-ray(EDX)spectroscopy,Fourier transform infrared(FTIR)spectroscopy,transmission electron microscopy(TEM)and X-ray diffraction(XRD),and the antibacterial activity was measured.The results showed that selenium salt was reduced into elementary selenium,and hydrogen bond-like interactions existed between the O–H groups of polyphenols and nanoselenium atoms.Additionally,TP-SeNPs were stable as an amorphous solid which was nearly spherically dispersed.The inhibitory effect of TP-SeNPs was better against Gram-positive bacteria than against Gram-negative bacteria.The minimum inhibitory concentration(MIC)against Listeria monocytogene was 0.06 mg/mL.TP-SeNPs effectively inhibited the formation of L.monocytogene biofilms,and destroyed the permeability and integrity of the cell wall and membrane,and caused leakage of cell contents,thereby leading to the death of L.monocytogene.This study offers a new idea for the application of blueberry pomace and selenium nanoparticles,and provides a theoretical basis for the development of selenium nanoparticles with antimicrobial activity.

关 键 词：蓝莓果渣多酚 多酚-纳米硒 结构表征 抑菌活性 

分 类 号：TS201.4[轻工技术与工程—食品科学]