核酸等温扩增技术检测动物源食品研究进展  

Research progress in nucleic acid isothermal amplification technology for detecting animal derived foods

作　　者：肖付刚[1,2,3] 张尧轩 谷蒙林 王德国 张国治[2] XIAO Fu-gang;ZHANG Yao-xuan;GU Meng-lin;WANG De-guo;ZHANG Guo-zhi(Food and Pharmacy College,Xuchang University,Xuchang 461000,Henan,China;College of Food Science and Engineering,Henan University of Technology,Zhengzhou 450000,Henan,China;Henan Key Laboratory of Biomarker Based Rapid-detection Technology for Food Safety,Xuchang 461000,Henan,China)

机构地区：[1]许昌学院食品与药学院,河南许昌461000 [2]河南工业大学粮油食品学院,河南郑州450000 [3]河南省食品安全生物标识快检技术重点实验室,河南许昌461000

出　　处：《粮食与油脂》2023年第12期22-28,共7页Cereals & Oils

基　　金：国家自然科学基金面上项目(32172300);国家重点研发计划子课题(2016YFD0500704-4);河南省科技攻关项目(212102310912);河南省高等学校重点科研项目(21B550006)。

摘　　要：综述了依赖核酸序列的扩增(NASBA)、链替代扩增(SDA)、滚环扩增(RCA)、环介导等温扩增(LAMP)、赖解旋酶恒温基因扩增(HDA)、重组酶聚合酶扩增(RPA)、梯型熔解温度等温扩增(LMTIA)几种主要核酸等温扩增检测技术的发展及其基本原理、特点和在动物源食品检测中的应用,并对几种主要核酸等温扩增检测技术的特点及应用进行比较。The development,basic principles,characteristics,and application of several main nucleic acid isothermal amplification detection technologies,including nuclear acid sequence-based amplification(NASBA),strand displacement amplification(SDA),rolling circle amplification(RCA),loop-mediated isothermal amplification(LAMP),helicase-dependent amplification(HDA),recombinase polymerase amplification(RPA),and ladder-shape melting temperature isothermal amplification(LMTIA)were re-viewed.And the characteristics and applications of several major nucleic acid isothermal amplification de-tection technologies were compared.

关 键 词：核酸等温扩增技术 检测 动物源食品 梯型熔解温度等温扩增技术 

分 类 号：TS207[轻工技术与工程—食品科学]