油橄榄果提取物体外降脂活性成分筛选及作用机制探究  

Screening of in vitro lipid-lowering active ingredients from Olea europaea L.fruit extract and preliminary study on the mechanism

作　　者：张鑫 徐芳华 袁奖娟 邱国玉 王小芳 裴栋[2,4] 王宁丽 阚欢[1] ZHANG Xin;XU Fang-Hua;YUAN Jiang-Juan;QIU Guo-Yu;WANG Xiao-Fang;PEI Dong;WANG Ning-Li;KAN Huan(College of Life Science,Southwest Forestry University,Kunming 650224,China;CAS Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province,Lanzhou Institute of Chemical Physics,Chinese Academy of Sciences,Lanzhou 730000,China;Gansu Pharmaceutical Group Science and Technology Innovation Research Institute Co.,Ltd.,Lanzhou 730000,China;Yunnan Olive Health Industry Innovation Research and Development Co.,Ltd.,Lijiang 674100,China)

机构地区：[1]西南林业大学生命科学学院,昆明650224 [2]中国科学院兰州化学物理研究所中国科学院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室,兰州730000 [3]甘肃药业集团科技创新研究院有限公司,兰州730000 [4]云南油橄榄大健康产业创新研究发展有限公司,丽江674100

出　　处：《食品安全质量检测学报》2023年第22期268-277,共10页Journal of Food Safety and Quality

基　　金：甘肃省科技重大专项(22ZD6FA021);兰州市科技计划项目(2022-2-2);云南省重点研发计划项目(202203AD150003);云南省重大科技专项计划项目(202302AE090007);2021年度兰州市人才创新创业项目(2021-RC-124)。

摘　　要：目的 探究油橄榄果提取物体外降脂活性物质基础,并初步探讨活性成分在体内的降脂作用机制。方法 建立17个油橄榄果提取物样品高效液相色谱(high performance liquid chromatography, HPLC)叠加图谱,以牛磺胆酸钠结合能力评价各样品的降脂活性,通过建立样品共有峰与降脂活性的谱效关系,筛选潜在的降脂活性成分;并应用计算机虚拟筛选技术预测靶蛋白,结合分子对接预测其作用靶点,初步研究其降脂机制。结果 17个油橄榄果提取物样品均具有不同程度降脂能力,峰P6、P10、P13、P14、P15、P17、P19和P20为潜在的降脂活性成分,其中P6和P13鉴定为羟基酪醇和断氧化马钱子苷,二者分别与多不饱和脂肪酸脂氧合酶ALOX15和钠/葡萄糖共转运蛋白1的残基存在氢键、疏水相互作用及静电相互作用,进而发挥结合抑制作用。结论 油橄榄果提取物中活性成分不仅能通过与牛磺胆酸钠结合,而且还能与靶蛋白结合发挥降脂作用,具有多成分、多靶点、多途径的特点,本研究结果可为后续研究提供理论依据。Objective To explore the active substance basis of in vitro lipid-lowering activity of Olea europaea L.fruit extract,and preliminarily investigate the mechanism of lipid-lowering action of active ingredient.Methods High performance liquid chromatography(HPLC)fingerprints of 17 Olea europaea L.fruit extract samples were established.The lipid-lowering activity of each sample according to the binding ability of sodium taurocholic acid was evaluated.The spectral relationship between the common peaks and lipid-lowering activity was established to screen the potential lipid-lowering active ingredients.Computer virtual screening technology was used to predict the target protein,and molecular docking was used to predict the target of action,and the mechanism of lipid-lowering was preliminarily studied.Results The 17 samples all had different lipid-lowering abilities.The P6,P10,P13,P14,P15,P17,P19 and P20 were potential lipid-lowering active components,and P6 and P13 were identified as hydroxytyrosol and secoxyloganin.They had hydrogen bonding,hydrophobic interaction and electrostatic interaction with residues of polyunsaturated fatty acid lipoxygenase ALOX15 and sodium/glucose cotransporter 1,respectively,and thus exerted binding inhibition.Conclusion The active ingredient in Olea europaea L.fruit extract can not only bind with sodium taurocholate,but also bind with target protein to play a lipid-lowering effect,which has the characteristics of multi-component,multi-target and multi-pathway,the results of this study can provide theoretical basis for subsequent research.

关 键 词：油橄榄果 谱效关系 羟基酪醇 断氧化马钱子苷 分子对接 

分 类 号：TS201.4[轻工技术与工程—食品科学]