响应面法优化木聚糖酶的修饰工艺  

Optimization of xylanase modification process by response surface method

作　　者：赵烊 郑明珠[1,2] 刘景圣 ZHAO Yang;ZHENG Ming-zhu;LIU Jing-sheng(College of Food Science and Engineeing,Jilin Agricultural University,Changchun 130118,Jilin,China;National Engineering Research Center for Wheat&Corn Further Processing,Changchun 130118,Jilin,China)

机构地区：[1]吉林农业大学食品科学与工程学院,吉林长春130118 [2]小麦和玉米深加工国家工程研究中心,吉林长春130118

出　　处：《粮食与油脂》2024年第1期122-127,共6页Cereals & Oils

基　　金：国家重点研发计划项目(2021YFD2101000、2021YFD2101001)。

摘　　要：探究化学修饰对木聚糖酶(XY)相对活性的影响,以XY相对活性为指标,利用丁二酸酐、乙酸酐、马来酸酐(MA)和邻苯二甲酸酐对XY进行化学修饰。采用单因素试验筛选出最优酸酐修饰剂,并进行响应面法优化酸酐修饰条件。结果表明:MA为最优修饰酸酐,最佳修饰条件为修饰温度14℃、修饰时间4h、pH7.0、MA添加量40%(以XY质量计)。在此条件下,马来酸酐修饰木聚糖酶(MA-XY)的相对活性为154.62%±1.24%。To explore the effect of chemical modification on xylanase(XY)relative activity,XY was chemical modified with succinic anhydride,acetic anhydride,maleic anhydride(MA)and phthalic an-hydride based on XY relative activity.The optimal anhydride modifier was selected by single factor test,and the modification conditions were optimized by response surface methodology.The results showed that MA was the optimal modification anhydride,and the optimal modification conditions were as follows:modification temperature 14℃,modificationtime4h,pH 7.0,and MA addition40%(basedonXY mass).Under these conditions,the relative activity of maleic anhydride modified xylanase(MA-XY)was 154.62%±1.24%.

关 键 词：工艺优化 木聚糖酶 酸酐 化学修饰 

分 类 号：TS201.1[轻工技术与工程—食品科学]