肉制品中致泻大肠埃希菌的分子特征分析  

Molecular characterization of Diarrheagenic Escherichia coli in meat products

作　　者：姬庆龙 赵贵明[1] 杨海荣[1] 王娉[1] 赵勇胜[1] 赵晓美[1] 陈颖[1] JI Qinglong;ZHAO Guiming;YANG Hairong;WANG Ping;ZHAO Yongsheng;ZHAO Xiaomei;CHEN Ying(Chinese Academy of Inspection and Quarantine,Beijing 100176,China)

机构地区：[1]中国检验检疫科学研究院,北京100176

出　　处：《中国食品卫生杂志》2023年第9期1272-1279,共8页Chinese Journal of Food Hygiene

基　　金：中国检验检疫科学研究院基本科研业务费项目(2020JK011)。

摘　　要：目的了解国内肉制品中致泻大肠埃希菌(DEC)的流行特征。方法运用全基因组测序技术对86株DEC进行分子特征分析,明确优势病理类型、序列型和血清型。通过全基因组单核苷酸多态性分析,确定我国肉制品来源DEC菌株之间的系统发育关系。结果肉制品中致泻大肠埃希菌的病理类型以EAEC为主,86株DEC可分为8种毒力基因型,包括48个ST型(包括6个新ST型)和55个O:H血清型;ST11和CC10为优势序列型和克隆复合体,O157、O15和O6为优势血清群。DEC菌株间表现为高度的遗传异质性,相同病理类型的同源性较低,处在不同的进化分支上。结论DEC的病理类型与ST型及血清型间未见对应关系,通过对ST型及血清型的监测不能直接对病理类型进行判断,但数量最多的ST型及血清型揭示了DEC的主要危害特征与防控点。可根据全基因组测序结果补充和优化DEC的判定方法,为今后DEC的溯源及流行病学调查提供数据支撑。Objective This study aimed to understand the epidemic characteristics of Diarrheagenic Escherichia coli(DEC)in domestic meat products.Methods The molecular characteristics of 86 strains of DEC were analyzed by whole genome sequencing technology,and the dominant pathological types,STs,and serotypes were identified.The phylogenetic relationship between DEC strains derived from meat products in China was determined through whole genome single nucleotide polymorphism.Results The results showed that the pathological types of DEC in meat products were mainly EAEC.Eighty-six DEC strains were divided into eight virulence genotypes,including 48 ST types(including six new ST types),and 55 O:H serotypes.ST11 and CC10 were the predominant ST and clonal complex,respectively.O157,O15,and O6 were the predominant serogroups.DEC strains exhibited high genetic heterogeneity,the same pathological type had low homology,and they were in different evolutionary branches.Conclusion There is no correspondence between DEC pathological types and ST types and serotypes.The pathological types cannot be directly judged by monitoring ST types and serotypes,but the most numerous ST types and serotypes reveal the main hazard characteristics and prevention of DEC.The determination method of DEC can be supplemented and optimized according to the results of whole genome sequencing,which can provide data support for the traceability and epidemiological investigation of DEC in the future.

关 键 词：致泻大肠埃希菌 毒力基因 多位点序列分型 血清型 

分 类 号：R155[医药卫生—营养与食品卫生学]