NaCl对可口革囊星虫体壁肌原纤维蛋白理化和凝胶性质的影响  

Effects of NaCl on Physicochemical and Gel Properties of Myofibrillar Protein from the Body Wall of Phascolosoma esculenta

作　　者：方佳琪 薛茜 王芳 陈朝阳 蔡荣杰 郭凤仙 汤璧蔚 黄慧 Fang Jiaqi;Xue Xi;Wang Fang;Chen Chaoyang;Cai Rongjie;Guo Fengxian;Tang Biwei;Huang Hui(Fujian Province Key Laboratory for the Development of Bioactive Material from Marine Algae,Quanzhou 362000,Fujian;College of Oceanology and Food Science,Quanzhou Normal University,Quanzhou 362000,Fujian;College of Food Science,Fujian Agriculture and Forestry University,Fuzhou 350002;Quanzhou Wushugong Food CoPeMPany Limited,Quanzhou 362700,Fujian)

机构地区：[1]福建省海洋藻类活性物质制备与功能开发重点实验室,福建泉州362000 [2]泉州师范学院海洋与食品学院,福建泉州362000 [3]福建农林大学食品科学学院,福州350002 [4]泉州五叔公食品有限责任公司,福建泉州362700

出　　处：《中国食品学报》2023年第12期77-86,共10页Journal of Chinese Institute Of Food Science and Technology

基　　金：2021年国家大学生创新创业训练计划项目(202110399014)。

摘　　要：通过对可口革囊星虫体壁肌原纤维蛋白(PeMP)的总蛋白溶解性、不同种类蛋白溶解性、紫外吸收光谱、内源荧光光谱、Zeta电位、傅里叶变换红外光谱(FTIR)、巯基含量、表面疏水性、变性温度和凝胶结构的分析,研究NaCl对PeMP的理化和凝胶性质的影响。结果表明:随着NaCl浓度的增加,PeMP的溶解性增大,PeMP中副肌球蛋白和肌球蛋白含量显著增加。PeMP的紫外吸收光谱及其紫外吸收光谱的相应二阶导数光谱和PeMP的内源荧光光谱的最大吸收波长(λ_(max))均发生红移。PeMP的Zeta电位增大。FTIR分析表明PeMP的二级结构发生转变,β-折叠逐渐增加,而α-螺旋逐渐降低。PeMP的总巯基和非蛋白巯基含量降低,而分型巯基含量升高,表面疏水性增大。PeMP的变性温度降低。PeMP凝胶的蛋白分子间的排列更为紧密。研究结果为改良可口革囊星虫体壁凝胶性质,制备高品质“土笋冻”产品奠定基础。In order to investigate the effects of NaCl on the physicochemical and gel properties of myofibrillar protein(PeMP)from the body wall of Phascolosoma esculenta Chen et.Yeh,the determination and observation results of solubility of total PeMP,solubility of different types of proteins from PeMP,ultraviolet absorption spectrum,endogenous fluorescence spectrum,Zeta potential,Fourier transform infrared spectrum(FTIR),sulfhydryl group contents,surface hydrophobicity,denaturation temperature and gel structure were analyzed.The results showed that the solubility of PeMP increased,the contents of paramyosin and myosin increased significantly,with the increase of NaCl concentration.The maximum absorption wavelength(λmax)of the ultraviolet absorption spectrum of PeMP,of the corresponding second derivative spectrum of ultraviolet absorption spectrum of PeMP and of the endogenous fluorescence spectrum of PeMP was redshifted,respectively.The Zeta potential of PeMP increased.FTIR analysis showed that the secondary structure of PeMP changed,theβ-sheet increased,while theα-helix decreased gradually.The contents of total and non-protein sulfhydryl groups decreased in PeMP,but the contents of typing sulfhydryl group increased,and the surface hydrophobicity of PeMP increased.The denaturation temperature of PeMP decreased.Protein molecules of PeMP gel were more tightly packed.These results will lay a foundation for the improvement of the gel of body wall from P.esculenta and the preparation of high-quality'earth bamboo shoot jelly'products.

关 键 词：可口革囊星虫 肌原纤维蛋白 理化性质 凝胶性质 氯化钠 

分 类 号：TS254.1[轻工技术与工程—水产品加工及贮藏工程]