TG酶/k-卡拉胶协同改性明胶复合膜的工艺优化及表征  

Process Optimization and Characterization of Gelatin Composite Film Modified by TGase Associated with k-Carrageenan

作　　者：应天昊 朱芸杉 胡杨[1] YING Tian-hao;ZHU Yun-shan;HU Yang(College of Food Science and Technology,Huazhong Agricultural University,Wuhan 430070,China)

机构地区：[1]华中农业大学食品科学与技术学院,武汉430070

出　　处：《中国调味品》2024年第2期10-18,27,共10页China Condiment

基　　金：国家十四五重点研发项目(2022YFD2100904);湖北省自然科学基金项目(ZRMS2022000745)。

摘　　要：为了研制一种性能优良的天然保鲜膜,该研究以明胶为基质,通过k-卡拉胶与谷氨酰胺转氨酶(TG酶)对明胶进行协同改性,采用流延法制备一种新型明胶复合膜,通过单因素试验与正交试验研究k-卡拉胶添加量、TG酶添加量、TG酶反应时间和干燥温度对明胶复合膜物理性能的影响。得出最佳制备工艺为明胶添加量5%、k-卡拉胶添加量0.5%、TG酶添加量0.2%、反应时间60 min、干燥温度45℃。在最佳工艺条件下制备的明胶复合膜拉伸强度为14.33 MPa,断裂伸长率为44.24%,水溶性为26.72%,水蒸气透过系数为0.278 g·mm/(kPa·h·m^(2))。优化制备工艺后,利用X射线衍射、热重分析等手段对不同明胶复合膜的结构进行表征。表征结果表明,k-卡拉胶与TG酶分别通过静电相互作用与氨基酸异肽键对明胶进行协同改性。该研究为新型明胶复合膜的开发提供了理论依据。In order to develop a natural preservative film with excellent performance,in this study,with gelatin as the substrate,k-carrageenan and transglutaminase(TGase)are used to modify gelatin,and a new gelatin composite film is prepared by tape casting method.The effects of the addition amount of k-carrageenan,the addition amount of TGase,the reaction time of TGase and the drying temperature on the physical properties of gelatin composite film are investigated by single factor test and orthogonal test.It is determined that the optimum preparation process is 5%gelatin,0.5%k-carrageenan,0.2%TGase,reaction time 60 min and drying temperature 45℃.The tensile strength of the gelatin composite film prepared under the optimum process conditions is 14.33 MPa,the elongation of break is 44.24%,the water solubility is 26.72%and the water vapor permeability coefficient is 0.278 g·mm/(kPa·h·m^(2)).After the optimization of preparation process,the structures of different gelatin composite films are characterized by X-ray diffraction and thermogravimetric analysis.The characterization results show that k-carrageenan and TGase synergistically modify gelatin through electrostatic interaction and amino acid isopeptide bonds respectively.This study has provided a theoretical basis for the development of new gelatin composite film.

关 键 词：明胶 K-卡拉胶 TG酶 复合膜 协同改性 

分 类 号：TS201.7[轻工技术与工程—食品科学]