超声辅助提取花椒精油及抗氧化活性分析  

Ultrasonic-Assisted Extraction of Essential Oil from Zanthoxylum bungeanum Maxim.and Analysis of Its Antioxidant Activity

作　　者：张莹丽 柳亚文 杨红应 严露露 冯世杰 陈丽文 ZHANG Ying-li;LIU Ya-wen;YANG Hong-ying;YAN Lu-lu;FENG Shi-jie;CHEN Li-wen(School of Horticulture,Xinyang Agriculture and Forestry University,Xinyang 464000,China;The Library of Xinyang Agriculture and Forestry University,Xinyang 464000,China)

机构地区：[1]信阳农林学院园艺学院,河南信阳464000 [2]信阳农林学院图书馆,河南信阳464000

出　　处：《中国调味品》2024年第2期186-191,共6页China Condiment

基　　金：河南省科技厅科技攻关项目(172102310445);河南省本科高校省级大学生创新创业训练计划项目(S202110480035);信阳农林学院设施作物栽培关键技术及其装备智能化研究科技创新团队(XNKJTD-011)。

摘　　要：文章采用超声辅助水蒸气蒸馏法提取花椒精油,通过单因素试验和正交试验确定提取花椒精油的最佳工艺条件为原料粒度50目、料液比1∶15、超声温度50℃、超声时间60 min,在此条件下精油得率达2.61%。用气相色谱-质谱联用仪分析最佳工艺条件下提取的精油的化学成分,结果显示花椒精油主要含柠檬烯(18.267%)、4-萜烯醇(10.625%)、月桂烯(10.009%)、α-松油醇(8.199%)、乙酸松油酯(6.499%)、3-莰烯(6.512%)等。抗氧化活性研究结果表明,花椒精油对DPPH自由基和超氧阴离子自由基有很好的清除效果,在试验浓度范围内最高清除率分别可达到72.5%和80.6%。In this paper,the essential oil is extracted from Zanthoxylum bungeanum Maxim.by ultrasonic-assisted steam distillation method.Through single factor test and orthogonal test,the optimum process conditions for the extraction of essential oil from Zanthoxylum bungeanum Maxim.are determined as follows:the particle size of the raw material is 50 mesh,the ratio of solid to liquid is 1∶15,the ultrasonic temperature is 50℃and the ultrasonic time is 60 min.The yield of essential oil reaches 2.61%under such conditions.The chemical components of the essential oil extracted under the optimum process conditions are analyzed by gas chromatograph-mass spectrometer.The results show that the essential oil from Zanthoxylum bungeanum Maxim.mainly contains limonene(18.267%),4-terpenol(10.625%),myrcene(10.009%),α-terpineol(8.199%),terpinyl acetate(6.499%)and 3-camphene(6.512%).The research results of antioxidant activity show that Zanthoxylum bungeanum Maxim.essential oil has good scavenging effects on DPPH free radicals and superoxide anion free radicals,and the highest scavenging rates could reach 72.5%and 80.6%respectively in the test concentration range.

关 键 词：超声辅助提取 花椒精油 提取工艺 抗氧化活性 

分 类 号：TS264.3[轻工技术与工程—发酵工程]