ε-聚赖氨酸微生物生产及其应用研究进展  

Recent advances in microbial production ofε-poly-L-lysine and its applications

作　　者：庄孝东 白森萌 李博彦 胡翠英[1] 王靖[1] 英晓莉 鞠鑫 扶教龙[1] 李良智[1] Zhuang Xiaodong;Bai Senmeng;Li Boyan;Hu Cuiying;Wang Jing;Ying Xiaoli;Ju Xin;Fu Jiaolong;Li Liangzhi(College of Chemical and Life Sciences,Suzhou University of Science and Technology,Suzhou 215009)

机构地区：[1]苏州科技大学化学与生命科学学院,苏州215009

出　　处：《中国抗生素杂志》2023年第10期1081-1095,共15页Chinese Journal of Antibiotics

基　　金：国家自然科学基金(No.21676173);苏州科技大学科研基金资助项目(No.XKZ201411)。

摘　　要：ε-聚赖氨酸是一种同聚酰胺生物聚合物,由25~35个L-赖氨酸残基通过α-羧基和ε-氨基缩合形成的酰胺键连接而成。因其独特的结构,ε-聚赖氨酸具有许多优异的性能,如可食性、水溶性、热稳定性、可降解性、广谱抗菌活性和无毒性等。因此,ε-聚赖氨酸已在日本、韩国、美国和中国等国家被广泛用作食品防腐剂。目前,ε-聚赖氨酸主要由白色链霉菌发酵生产。自ε-聚赖氨酸于1977年由日本学者发现以来,ε-聚赖氨酸发酵生产得到了很大提高,对其应用研究也由最初的食品领域拓展到了许多其他的领域。本文首先概述了ε-聚赖氨酸生物合成机理、菌种选育和发酵生产策略,在简要介绍其抑菌机制基础上,着重介绍了ε-聚赖氨酸在食品、医学和材料等领域应用研究进展,最后,对相关研究进行了展望,以期为ε-聚赖氨酸微生物生产及其应用研究提供有益的借鉴。ε-poly-L-lysine(ε-PL)is a natural homo-polyamide biopolymer consisting of 25-35 L-lysine residues with the amide linkages formed between theε-amino andα-carboxyl groups.Due to its unique structure,ɛ-PL has many excellent properties,such as edibility,water solubility,thermostability,biodegradability,broad-spectrum antibacterial activity,and nontoxicity.Therefore,ε-PL has been widely used as a food preservative in Japan,Korea,the United States,China,and other countries.At present,ε-PL is mainly produced by the aerobic fermentation of Streptomyces albulus.Sinceε-PL was discovered by Japanese scholars in 1977,the fermentation production ofε-PL has been greatly improved,and the application ofε-PL has also expanded from the initial food field to many other fields.This paper firstly outlined the biosynthesis mechanism,strain selection,and fermentation production strategy ofε-PL,and highlighted the progress of research onε-PL in food,medical and material applications based on its bacterial inhibition mechanism.Finally,an outlook on related research is given to provide a useful reference for the microbial production ofε-PL and its application research.

关 键 词：Ε-聚赖氨酸 微生物生产 抗菌机制 应用研究 白色链霉菌 

分 类 号：R978.1[医药卫生—药品]