纳米花固定化蔗糖酶的研究  

Study on immobilization of sucrase by nano-flower

作　　者：崔诗琦 姜晓冬 王世杰 王红英[1] 钱斯日古楞 CUI Shiqi;JIANG Xiaodong;WANG Shijie;WANG Hongying;QIAN Siriguleng(School of Biological Engineering,Dalian Polytechnic University,Dalian 116034,China)

机构地区：[1]大连工业大学生物工程学院,大连116034

出　　处：《生物学杂志》2024年第1期113-119,共7页Journal of Biology

基　　金：内蒙古自治区科技重大专项项目(222089)。

摘　　要：蔗糖酶(Sucrase,EC 3.2.1.26)在工业上具有较高的经济价值,可用于分解蔗糖生成果糖和葡萄糖,现今在工业化技术上存在着蔗糖酶生产果糖方法单一、活力不易保持且难以重复利用等问题。采用共沉淀法制备Mn_(3)(PO_(4))_(2)-蔗糖酶的纳米花固定化酶[以下简称Sucrase@Mn_(3)(PO_(4))_(2)],并对制备条件进行优化;同时对固定化酶的形态、结构进行表征,对固定化酶的动力学特性进行分析。结果表明:制得的Sucrase@Mn_(3)(PO_(4))_(2)纳米花颗粒分散,大小均匀,呈雏菊形、盛开状,且内部晶体结构完整;固定化酶有明显的Mn^(2+)、PO^(3-)_(4)、酶蛋白质的EDS特征峰及明显的酶蛋白质和磷酸根的FT-IR吸收特征;固定化酶表现出良好的温度稳定性和酸碱稳定性;Sucrase@Mn_(3)(PO_(4))_(2)被重复使用7次后其活性仍能保持其最初活力的40.38%;固定化酶在4℃保存28 d时仍保持40%的活性。Sucrase(Sucrase,EC 3.2.1.26)has high economic value in industry,and can be used to decompose sucrose to produce fructose and glucose.Nowadays,there are problems such as single sucrase fructose production method,not easy to maintain vitality and difficult to reuse.The nano-flower immobilized enzyme of Mn_(3)(PO_(4))_(2)/sucrase\was prepared by co-precipitation method,and the preparation conditions were optimized.The morphology and structure of the immobilized enzyme were characterized and the kinetic characteristics of the immobilized enzyme were analyzed.The results showed that the nano-flower particles of Sucrase@Mn_(3)(PO_(4))_(2)were uniform in size,dispersed,daisy-shaped and blooming.The internal crystal structure of nano-flower immobilized enzyme was complete.The immobilized enzyme had obvious EDS characteristic peaks of Mn^(2+),PO^(3-)_(4)and enzyme protein,and had obvious FT-IR absorption characteristics of enzyme protein and phosphoric acid.It showed good temperature stability and acid-base stability,and maintained its original activity of 40.38%after repeated use for 7 times and 40%when stored at 4℃for 28 days.

关 键 词：固定化酶 纳米花 蔗糖酶 酶学性质 酶活力 

分 类 号：Q556[生物学—生物化学]