新疆和田地区人源肠道乳酸菌的筛选及其体外益生特性分析  

Probiotic Characteristics of Lactic Acid Bacteria Isolated from the Intestinal Tract of Mothers and Infants in Hotan,Xinjiang

作　　者：李建军 古丽拜克热木·艾比卜拉 倪永清[1] 李谞 LI Jianjun;Gulibaikeremu·AIBIBOLA;NI Yongqing;LI Xu(College of Food Science and Technology,Shihezi University,Shihezi 832003,China)

机构地区：[1]石河子大学食品学院,新疆石河子832003

出　　处：《食品工业科技》2024年第5期134-143,共10页Science and Technology of Food Industry

基　　金：国家自然科技基金地区项目(32260567);第八师石河子市科技专项(2020PT01);新疆生产建设兵团科技创新团队(2020CB007);石河子大学创新发展专项(CXFZ202109)。

摘　　要：本研究以新疆和田地区维吾尔族母婴粪便为样品源,对其中乳酸菌(Lactic acid bacteria)进行分离鉴定。测定乳酸菌菌株对腹泻致病菌抑菌性能,及抑菌乳酸菌菌株的体外益生特征实验。依据groEL功能基因测序从19对母婴样本分离出属于15个种的113株乳酸菌,其中优势种是唾液联合乳杆菌(Ligilactobacillus salivarius)和香肠伴生乳杆菌(Companilactobacillus farciminis)。从113株乳酸菌菌株中筛选得到29株菌株对6种腹泻致病菌都有抑制效果,其中,香肠伴生乳杆菌HTb36X-2通过4 h的模拟胃肠液处理后存活率最高达69.50%;罗伊氏粘液乳杆菌(Limosilactobacillus reuteri)HTb34X5-6、HTb34X5-1自聚性最高与疏水性最高,其疏水性分别为31.67%和29.00%,自聚性分别为81.32%和70.91%;并结合抑菌菌株的抗生素耐药性和碳源代谢能力,最终筛选出香肠伴生乳杆菌HTb36X-2,副干酪乳酪杆菌(Lacticaseibacillus paracasei)HTb6X-6,干酪乳酪杆菌(Lacticaseibacillus casei)17X-4、17X-5,罗伊氏粘液乳杆菌HTb34X-5-1、HTb34X-5-6,发酵粘液乳杆菌(Limosilactobacillus fermentum)10D12-2共7株作为潜在益生菌菌株,为开发具有抗腹泻功能的优良益生菌株及产品奠定前期基础。In this study,the lactic acid bacteria from the feces of Uygur mother-infant pairs in Hotan,Xinjiang,was isolated and identified.The bacteriostatic performance of the isolates for diarrhea pathogenic bacteria was assessed,and the probiotic properties were investigated in vitro.According to the groEL gene sequencing,113 isolates of lactic acid bacteria representing 15 species were isolated from 19 mother-infant pairs,with Ligilactobacillus salivarius and Companilactobacillus farciminis being the dominant species,and 29 strains had inhibition effect on all the 6 pathogenic bacteria were screened from all strains.Among the strains with antibacterial activity,Companilactobacillus farciminis HTb36X-2 had the highest survival rate of 69.50%after being treated with simulated gastrointestinal juices for 4 h.Limosilactobacillus reuteri HTb34X5-6 and HTb34X5-1 had the highest auto-aggregation and hydrophobicity,with the hydrophobicity of 31.67%and 29.00%,and auto-aggregation of 81.32%and 70.91%,respectively.Combined with antibiotic resistance and carbohydrate metabolism of all 29 strains,a total of 7 strains of Companilactobacillus farciminis HTb36X-2,Lacticaseibacillus paracasei HTb6X-6,Lacticaseibacillus casei 17X-4,17X-5,Limosilactobacillus reuteri HTb34X-5-1,HTb34X-5-6,Limosilactobacillus fermentum 10D12-2 were screened as potential probiotics,which might take a foundation for the development of probiotic and products with anti-diarrhea function.

关 键 词：乳酸菌 GROEL 抑菌活性 益生特性 益生菌 

分 类 号：TS252.1[轻工技术与工程—农产品加工及贮藏工程]