脱支玉米淀粉-壳聚糖复合物的制备及结构表征  

Preparation and Structural Characterization of Debranched Maize Starch-chitosan Complex

作　　者：刘敏[1,2] 邢倩[1] 柯胜 周中凯 Liu Min;Xing Qian;Ke Sheng;Zhou Zhongkai(College of Food Science and Engineering,Tianjin University of Science and Technology,Tianjin 300457;Key Laboratory of Agricultural Product Processing and Quality Control of Specialty,Shihezi University,Shihezi 832003,Xinjiang)

机构地区：[1]天津科技大学食品科学与工程学院,天津300457 [2]石河子大学、农产品加工与质量安全控制重点实验室,新疆石河子832003

出　　处：《中国食品学报》2024年第2期169-178,共10页Journal of Chinese Institute Of Food Science and Technology

基　　金：中央引导地方科技发展资金项目(22ZYGSN00360)。

摘　　要：为了探究脱支处理对玉米淀粉-壳聚糖复合物理化性质和流变特性的影响,以玉米淀粉(MS)为原料,用普鲁兰酶酶解脱支,将脱支淀粉(DBS)与壳聚糖(CS)溶液混合制备脱支玉米淀粉-壳聚糖复合物(DBS-CS),测定复合物的微观结构、粒径分布、结晶结构、分子短程有序结构、流变学特性。结果表明:脱支玉米淀粉-壳聚糖复合物的颗粒表面附着壳聚糖越多,颗粒间的结合力越紧密且复合物的平均粒径越大,热稳定性提高。脱支后的DBS和DBS-CS的结晶度降低,结晶结构由A型转变为B型。红外光谱和核磁共振氢谱表明淀粉与壳聚糖能够形成复合物且脱支后的玉米淀粉与壳聚糖复合效果更好。脱支淀粉-壳聚糖复合物具有典型的弱凝胶动态流变行为和假塑性剪切变稀行为。本研究结果为获得一种新型绿色的复合改性淀粉提供了理论依据。In order to investigate the effect of debranching treatment on the structural characteristics and physicochemical properties of maize starch-chitosan complex.In this study,maize starch(MS)was used as raw material,pullulanase was used for enzymatic debranching,debranched maize starch-chitosan complex(DBS-CS)was prepared by mixing debranched starch(DBS)and chitosan(CS)solution.The microstructure,particle size distribution,crystalline structure,molecular short-range ordered structure and rheological properties of the complex were determined.Results showed that the more chitosan attached to the particle surface of debranched maize starch-chitosan complex,the tighter the binding force between particles and the larger the average particle size of the complex,and improved thermal stability.The crystallinity of DBS and DBS-CS decreased after debranched,the crystal structure changed from A type to B type.The FT-IR spectrums of and 1H NMR spectroscopy showed that starch and chitosan could form complex,and the composite of debranched maize starch and chitosan had better effect.The debranched starch-chitosan complex had typical weak gel dynamic rheological behavior and pseudoplastic shear thinning behavior.This study provides a theoretical basis for obtaining a new type of green security complex modified starch.

关 键 词：玉米淀粉 壳聚糖 脱支处理 相互作用 流变学性质 

分 类 号：TS231[轻工技术与工程—粮食、油脂及植物蛋白工程]