贺兰山东麓葡萄酒中生物胺特征分析  

Characteristics of Biogenic Amines in Wines from the Eastern Foot of Helan Mountains

作　　者：李瑞雪 王泽岚 龚慧 谢芳 LI Ruixue;WANG Zean;GONG Hui;XIE Fang(Ningxia Food Testing Institute/Key Laboratory of Quality and Safety of Chinese Wolfberry and Wine for State Market Regulation,Yinchuan 750001,China)

机构地区：[1]宁夏回族自治区食品检测研究院/国家市场监管重点实验室(枸杞及葡萄酒质量安全),宁夏银川750001

出　　处：《中外葡萄与葡萄酒》2024年第2期48-55,共8页Sino-Overseas Grapevine & Wine

基　　金：宁夏市场监管厅科技项目(2022SJKY0012)。

摘　　要：为探究贺兰山东麓产区葡萄酒中生物胺的轮廓特点,建立同时测定葡萄酒中腐胺、组胺、亚精胺、色胺、尸胺、酪胺和2-苯乙胺的液相色谱-串联质谱法(LC-MS/MS),以揭示贺兰山东麓葡萄酒样品中生物胺含量水平。结果表明,各生物胺组分在对应线性范围内均呈现较好的线性关系(r>0.999),平均回收率为82.3%~106.7%,7种生物胺定量限为10~20μg·L^(-1),满足分析要求。数据分析显示,红葡萄酒、白葡萄酒和桃红葡萄酒的生物胺总量分别为0.39~15.70、0~3.18、0~3.48 mg·L^(-1)。红葡萄酒中生物胺含量大小排序为酪胺>亚精胺>腐胺>2-苯乙胺,而白葡萄酒和桃红葡萄酒中为亚精胺>腐胺>尸胺。通过分析生物胺指数得出,红葡萄酒生物胺含量高于白葡萄酒和桃红葡萄酒。在红葡萄酒中,‘赤霞珠’‘美乐’‘黑比诺’和‘蛇龙珠’葡萄酒中各生物胺含量略有差异,其中‘蛇龙珠’葡萄酒中酪胺含量明显高于其他生物胺成分。该研究为贺兰山东麓葡萄酒的生物胺监管和溯源提供了数据支撑。To investigate the profile of biogenic amines(BAs)in wines from the eastern foot of Helan Mountains,the liquid chromatography-tandem mass spectrometry(LC-MS/MS)method was established to simultaneously determine putrescine,histamine,spermidine,tryptamine,cadaverine,tyramine and 2-phenylethylamine in wines,and BAs content level of wines was revealed.As results showed,each BAs has good linearity with r>0.999,the average recoveries varied between 82.3%-106.7%.LOQs of seven BAs were 10-20μg•L^(-1).According to statistical analysis,total BAs in red wine,white wine and rose wine were 0.39-15.70 mg•L^(-1),0-3.18 mg•L^(-1) and 0-3.48 mg•L^(-1),respectively.Based on analysis of mean concentration of each BAs,it was found that CTyramine>CSpermidine>CPutrescine>C2-Phenylethylamine in red wine,and CSpermidine>CPutrescine>CCadaverine in both white wine and rose wine.The biogenic amine index(BAI)value of red wine was greater than that of both white wine and rose wine,the content of BAs in'Cabernet Sauvignon''Merlot''Pinot Noir'and'Cabernet Gernischet'wine were slightly different,the content of tyramine in'Cabernet Gernischet'wine was significantly higher than that of other BAs.The results of BAs in wines are useful for monitoring quality of wines and their traceability.

关 键 词：贺兰山东麓 葡萄酒 液相色谱-串联质谱法 生物胺 

分 类 号：TS262.61[轻工技术与工程—发酵工程]