CRISPR/Cas-等温扩增技术在食源性病原菌检测中的研究进展  

Research Progress on Clustered Regularly Interspaced Short Palindromic Repeats(CRISPR)/CRISPR-Associated Protein(Cas)-Isothermal Amplification in the Detection of Foodborne Pathogens

作　　者：张琪 庞立冬 苏群超 宋丹靓敏 杨鑫焱 姜毓君[1] 张微[1] ZHANG Qi;PANG Lidong;SU Qunchao;SONG Danliangmin;YANG Xinyan;JIANG Yujun;ZHANG Wei(Key Laboratory of Dairy Science,Ministry of Education,College of Food Science,Northeast Agriculture University,Harbin 150030,China)

机构地区：[1]东北农业大学食品学院,乳品科学教育部重点实验室,黑龙江哈尔滨150030

出　　处：《食品科学》2024年第7期310-321,共12页Food Science

基　　金：黑龙江省自然科学基金项目(ZD2021C004)。

摘　　要：食源性病原菌引起的食品安全事件引起广泛关注,为保证食品安全,采取有效的检测手段至关重要。新兴的簇状规则间隔短回文重复序列(clustered regularly interspaced short palindromic repeats,CRISPR)及其关联蛋白(CRISPR-associated protein,Cas)组成的CRISPR/Cas系统是广泛存在于细菌和古细菌中的一种获得性免疫系统,可高效、特异性识别并切割外源核酸,与传统技术相比在检测病原菌方面更为高效。本文基于CRISPR/Cas生物传感系统-等温扩增技术联用的相关研究进行综述,对CRISPR/Cas系统的分类、原理以及与等温扩增耦合后在食源性病原菌检测方面的研究进展进行概述,并对其在即时检测应用中的前景以及所面临的挑战进行深入探讨,以期为CRISPR/Cas生物传感系统的进一步发展提供参考。Food safety incidents caused by foodborne pathogens have attracted widespread attention.In order to ensure food safety,it is essential to adopt effective detection means.The emerging clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated proteins(Cas)system,consisting of CRISPR and Cas,is an acquired immune system widely found in bacteria and archaea,which efficiently and specifically recognizes and cleave exogenous nucleic acids and is more efficient than conventional techniques for detecting pathogenic bacteria.Based on a review of recent progress in research on CRISPR/Cas biosensing system coupled with isothermal amplification technology,this paper summarizes the classification and principle of CRISPR/Cas and recent progress in the application of CRISPR/Cas coupled with isothermal amplification in foodborne pathogen detection,and discusses the prospects and challenges for CRISPR/Cas coupled with isothermal amplification in the real-time detection of foodborne pathogens.We expect that this review will provide a reference for the further development of CRISPR/Cas biosensing system.

关 键 词：食源性病原菌 CRISPR/Cas系统 等温扩增 纳米材料 功能核酸 

分 类 号：TS207.4[轻工技术与工程—食品科学]