B-cell lymphoma-2 phosphorylation at Ser70 site-related autophagy mediates puerarin-inhibited the apoptosis of MC3T3-E1 cells during osteoblastogenesis  

作　　者：LI Xi LIN Xiangquan CHEN Dongdong LIU Hui 

机构地区：[1]the Third Clinical Medical College,Fujian Medical University [2]Department of Orthopedics,Fuzhou Second Hospital,Fuzhou 350007,China

出　　处：《Journal of Traditional Chinese Medicine》2024年第1期27-34,共8页中医杂志（英文版）

基　　金：Fujian Provincial Natural Science:Exploration of Crisis Warning Values for Intraoperative Electrophysiological Monitoring in High-risk Cervical Spondylosis Patients on the Brink of Paralysis(No.2019J01548);Fujian Provincial Clinical Medical Research Center for First Aid and Rehabilitation in Orthopaedic Trauma:Study on the Treatment of Osteoporosis(No.2020Y2014)。

摘　　要：OBJECTIVE:To explore the relationship between autophagy and apoptosis regulated by puerarin during osteoblastogenesis.METHODS:In this study,the effects of puerarin on the autophagic activity and apoptosis level of osteoblast precursors(MC3T3-E1 cells)was observed.Subsequently,the roles of puerarin on B-cell lymphoma-2(Bcl-2)phosphorylation at different sites in osteoblast precursors were observed.The effect of puerarin on the interaction between Bcl-2 and autophagy regulatory molecule or pro-apoptotic molecule was also investigated using Co-immunoprecipitation assays.In addition,the effect of puerarin on mitochondrial membrane potential of osteoblast precursors was also identified by mitochondrial membrane potential fluorescence probe assays.RESULTS:Our results showed that puerarin can promote the autophagic activity and apoptosis level of MC3T3-E1cells.In addition,puerarin promoted Bcl-2phosphorylation at Ser70 site,and the dissociation of Bcl-2-Beclin1 complex.Moreover,puerarin could enhance the binding of Bcl-2-Bcl-2-Associated X(Bax)complex in MC3T3-E1 cells.Furthermore,puerarin increased the mitochondrial membrane potential of MC3T3-E1 cells.CONCLUSIONS:Therefore,puerarin promotes Beclin1into autophagy flux through Bcl-2 phosphorylation at Ser70,thereby enhancing autophagy of osteoblast precursors,which mediates its anti-apoptotic role during osteoblastogenesis.Furthermore,the dissociation of Bcl-2-Beclin1 complex is conducive to the binding of Bcl-2-Bax complex,which resists the apoptosis of osteoblast precursors via the increased mitochondrial membrane potential.

关 键 词：PUERARIN OSTEOBLASTS AUTOPHAGY APOPTOSIS lymphoma B-CELL 

分 类 号：R285[医药卫生—中药学]