响应面法优化马齿苋总多酚提取工艺及其抗氧化活性分析  

Optimization of total polyphenols extraction process from Portulaca oleracea L.by response surface method and analysis of its antioxidant activity

作　　者：祝媛媛[1] 任晓聪[1] 刘元松 刘绮玲 曾令菲 鲁新环[2] ZHU Yuan-yuan;REN Xiao-cong;LIU Yuan-song;LIU Qi-ling;ZENG Ling-fei;LU Xin-huan(Department of Biological and Chemical Engineering,Zhixing College of Hubei University,Wuhan 430011,Hubei,China;College of Chemistry and Chemical Engineering,Hubei University,Key Laboratory for Synthesis and Application of Organic Functional Molecules,Ministry of Education,Wuhan 430062,Hubei,China)

机构地区：[1]湖北大学知行学院生物与化学工程学院,湖北武汉430011 [2]湖北大学化学化工学院,有机功能分子合成与应用教育部重点实验室,湖北武汉430062

出　　处：《粮食与油脂》2024年第4期119-123,共5页Cereals & Oils

基　　金：材料化学工程国家重点实验室开放项目(KL21-07)。

摘　　要：以马齿苋为原料,采用超声辅助提取马齿苋总多酚。以总多酚提取量为指标,在单因素试验的基础上通过响应面法优化提取工艺,并分析马齿苋总多酚的抗氧化活性。结果表明:最佳提取工艺为液料比30:1(mL/g)提取温度45℃、乙醇体积分数60%、提取时间40min,在此条件下马齿苋总多酚提取量为6.89mg/g。抗氧化试验表明,马齿苋总多酚具有较好的抗氧化活性。Using Portulaca oleracea L.as raw material,total polyphenols from Portulaca oleracea L.was extracted by ultrasonic assisted.Using the total polyphenols extraction amount as the indicator,the extraction process was optimized by response surface method based on single factor experiments,and the antioxidant activity of total polyphenols from Portulaca oleracea L.was analyzed.The results showed that the optimal extraction process was liquid to material ratio 30:1(mL/g),extraction temperature 45℃,ethanol volume fraction 60%,and extraction time 40 min.Under these conditions,the total polyphenols extraction amount of Portulaca oleracea L.was 6.89 mg/g.The antioxidant test showed that the total polyphenols of Portulaca oleracea L.had good antioxidant activity.

关 键 词：马齿苋 总多酚 提取工艺 抗氧化活性 

分 类 号：TS201.1[轻工技术与工程—食品科学]