苦瓜多糖的响应面法提取及其抗氧化抑菌研究  

Study on the extraction and antioxidant antibacterial activities of bitter gourd polysaccharides using response surface method

作　　者：乔勤勤 胡静德 董志明 张乐乐 刘艳红 季春艳 李双芳 刘生杰 QIAO Qinqin;HU Jingde;DONG Zhiming;ZHANG Lele;LIU Yanhong;JI Chunyan;LI Shuangfang;LIU Shengjie(College of Information Engineering,Fuyang Normal University,Fuyang Anhui 236041,China;School of Biology and Food Engineering,Fuyang Normal University,Fuyang Anhui 236037,China)

机构地区：[1]阜阳师范大学信息工程学院,安徽阜阳236041 [2]阜阳师范大学生物与食品工程学院,安徽阜阳236037

出　　处：《阜阳师范大学学报（自然科学版）》2024年第1期36-44,共9页Journal of Fuyang Normal University:Natural Science

基　　金：安徽省科学研究自然学科重点项目(2023AH052855);安徽省高校优秀青年人才支持计划一般项目(gxyq2022190);国家级大学生创新创业训练项目(202213619004);安徽省大学生创新创业训练计划项目(s20211361910、s20221361913);阜阳师范大学信息工程学院自然科学研究项目(FXG2021ZY02);阜阳师范大学信息工程学院青年人才重点项目(2021XGRCXM004);阜阳地方农产品食品工艺开发科研创新团队(FX2020KCT02)。

摘　　要：本研究以市售苦瓜为材料,采用果胶酶从苦瓜粉中提取多糖,探究酶解温度、酶解时间、果胶酶用量、p H对苦瓜多糖提取率的影响,通过单因素实验对苦瓜多糖的提取率进行测定,然后用响应面方法优化苦瓜多糖的提取工艺。以DPPH自由基、ABTS自由基清除能力为指标测定苦瓜多糖抗氧化能力,通过抑菌圈大小测定苦瓜多糖抑菌活性。在温度为54℃,酶解时间为86 min,果胶酶添加量为0.05 g,pH 4.86的环境下苦瓜多糖的提取率最高为14.74%,通过抗氧化试验可知苦瓜多糖对DPPH自由基、ABTS自由基有较好地清除能力,通过抑菌试验可知苦瓜多糖能有效抑制大肠杆菌及金黄色葡萄球菌的繁殖。This study used commercially available bitter gourd as the material and extracted polysaccharides from bitter gourd powder using pectinase.The effects of enzymatic hydrolysis temperature,hydrolysis time,pectinase dosage,and pH on the extraction rate of bitter gourd polysaccharides were investigated.The extraction rate of bitter gourd polysaccharides was de-termined through single factor experiments,and the extraction process of bitter gourd polysaccharides was optimized using re-sponse surface method.The antioxidant capacity of bitter gourd polysaccharides was measured using DPPH free radical and ABTS free radical scavenging ability as indicators,and the antibacterial activity of bitter gourd polysaccharides was determined by the size of the inhibition zone.It was found that the extraction rate of bitter gourd polysaccharides was the highest at 14.74%in an environment with a temperature of 54 C,an enzymatic hydrolysis time of 86 minutes,a pectinase addition of 0.05 g,and a pH of 4.86.Through antioxidant tests,it was found that bitter gourd polysaccharides have a good ability to scavenge DPPH and ABTS free radicals.Through antibacterial tests,it was found that bitter gourd polysaccharides can effectively inhibit the prolifer-ation of Escherichia coli and Staphylococcus aureus.

关 键 词：苦瓜多糖 响应面 抗氧化活性 抑菌活性 

分 类 号：TS245.9[轻工技术与工程—制糖工程]