低温等离子体关键活性粒子对大豆球蛋白结构及致敏性的影响  

Effects of the Free Radicals in Cold Plasma Treatment on the Structure and Allergenicity of Glycinin

作　　者：彭开锋 周迎雪 李晓娟 刘志伟[2] PENG Kaifeng;ZHOU Yingxue;LI Xiaojuan;LIU Zhiwei(Qianjin Pharmaceutical Co.Ltd.,Zhuzhou 412000,China;College of Food Science and Technology,Hunan Agricultural University,Changsha 410128,China)

机构地区：[1]株洲千金药业股份有限公司,湖南株洲412000 [2]湖南农业大学食品科学技术学院,湖南长沙410128

出　　处：《现代食品科技》2024年第3期163-171,共9页Modern Food Science and Technology

基　　金：湖南省高新技术产业科技创新引领计划项目(2022GK4014);湖南省教育厅优秀青年项目(277109)。

摘　　要：该文通过构建单独活性粒子(·OH、H_(2)O_(2))氧化体系和基于低温等离子体技术(CP)的活性粒子屏蔽体系(屏蔽·OH:叔丁醇;屏蔽H_(2)O_(2):MnO_(2))的对比研究,探究CP活性粒子(·OH、H_(2)O_(2))对大豆球蛋白结构及致敏性影响的特异性。结果表明:单独·OH氧化体系,大豆球蛋白与IgG/IgE结合能力均随·OH含量增加而逐渐降低至69.00%(20 mmol/L);CP+叔丁醇的·OH屏蔽氧化体系中,结合能力由59.45%(IgG)、52.50%(IgE)(40 kV、2 min),分别增加至67.95%(IgG)(100 mmol/L)和73.77%(IgE)(300 mmol/L)。单独H_(2)O_(2)氧化体系,大豆球蛋白与IgG/IgE结合能力均随H_(2)O_(2)含量增加而降至65.65%(IgG)、30.84%(IgE)(2.0 mmol/L),基于CP+MnO_(2)的H_(2)O_(2)屏蔽体系中,随着MnO_(2)添加量的增加,结合能力分别增加至91.22%(IgG)、73.90%(IgE)(25 mmol/L)。利用SDS-PAGE和内源荧光光谱分别表征蛋白一级结构和三级结构变化,结果表明,·OH通过改变大豆球蛋白一级和三级结构,而H_(2)O_(2)改变蛋白三级结构达到消减大豆球蛋白致敏性目的。该研究证实·OH和H_(2)O_(2)作为具有氧化作用的活性粒子参与CP处理对蛋白结构的氧化修饰。A comparative investigation was performed using oxidation systems comprising single active particles,namely·OH and H_(2)O_(2),and free radical scavenging systems based on cold plasma(CP)(·OH scavenging:CP+tertiary butanol;H_(2)O_(2) scavenging:CP+MnO_(2))to explore the specificity of different active particles in CP treatment(·OH and H_(2)O_(2))on the structure and antigenicity of glycinin.The IgG/IgE binding abilities of glycinin were found to gradually decrease to 69.00%as the·OH concentration increased up to 20 mmol/L in the·OH oxidation system.In the CP+tertiary butanol oxidation system without·OH,the IgG/IgE binding abilities of glycinin increased from 59.45%(IgG)and 52.50%(IgE)with CP treatment(40 kV,2 min)alone to 67.95%(IgG)(100 mmol/L tertiary butanol)and 73.77%(IgE)(300 mmol/L tertiary butanol),respectively.Similarly,the IgG/IgE binding abilities of glycinin decreased to 65.65%(IgG)and 30.84%(IgE)as the H_(2)O_(2) concentration increased to 2.0 mmol/L in the H_(2)O_(2) oxidation system.In the CP+MnO_(2) oxidation system without H_(2)O_(2),the binding abilities of glycinin increased to 91.22%(IgG)and 73.90%(IgE)as the MnO_(2) concentration increased to 25 mmol/L.Structural analysis based on SDS-PAGE and fluorescence spectra indicate that the·OH-induced primary and tertiary structure changes of glycinin are directly related to the reduction of the antigenicity of glycinin.Meanwhile,only the tertiary structure of glycinin was modified by H_(2)O_(2).Therefore,·OH and H_(2)O_(2) are key active particles in CP treatment for the oxidative modification of protein structures and its related antigenicity elimination.

关 键 词：低温等离子体 大豆球蛋白 活性粒子 致敏性 

分 类 号：TS201.21[轻工技术与工程—食品科学]