酪蛋白糖巨肽对花生过敏原免疫反应性的影响  

Effects of casein glycomacropeptide on the immunoreactivity of peanut allergens

作　　者：曾冰蕙 宋若琳 刘雅利 孟婷云 曾令玉 车会莲[1] ZENG Bing-Hui;SONG Ruo-Lin;LIU Ya-Li;MENG Ting-Yun;ZENG Ling-Yu;CHE Hui-Lian(Food Science and Nutrition Engineering College,China Agricultural University,Beijing 100083,China)

机构地区：[1]中国农业大学食品科学与营养工程学院,北京100083

出　　处：《食品安全质量检测学报》2024年第5期43-50,共8页Journal of Food Safety and Quality

基　　金：国家自然科学基金项目(31972185)。

摘　　要：目的 探究酪蛋白糖巨肽(casein glycomacropeptides,CGMP)与花生过敏原相互作用并降低其免疫反应性的潜力。方法 通过蛋白-蛋白分子对接技术探讨CGMP与Ara h1、Ara h2是否有相互作用的潜力。进一步通过混合水浴加热制备CGMP与花生蛋白的混合溶液(mixed solution of casein glycomacropeptides and peanut proteins,MCGP),建立MCGP致敏、花生蛋白激发的BALB/c小鼠模型,研究MCGP对花生过敏反应的影响。最后使用圆二色谱法研究酪蛋白糖巨肽与Ara h1、Ara h2的相互作用力及对其结构的影响。结果 CGMP与Ara h1、Ara h2间存在次级键(盐桥、氢键、范德华力),部分作用于过敏原表位;与花生蛋白过敏组相比, MCGP致敏组血清中的花生蛋白特异性免疫球蛋白E (specific immunoglobulin E, sIgE)、sIgG1、sIgG2a含量显著下降,白介素-4 (interleukin-4, IL-4)、IL-5、组胺水平显著下降,转化生长因子-β (transforming growth factor-β, TGF-β)、γ干扰素(interferon-gamma, IFN-γ)水平显著升高, MCGP中Ara h2的α-螺旋与β-折叠的比例改变。结论 CGMP能够改变Ara h2的结构,遮蔽花生过敏原表位,抑制sIgE、sIgG结合Ara h1、Ara h2,降低部分花生过敏原的免疫反应性。Objective To investigate the potential of casein glycomacropeptides(CGMP)to interact with peanut allergens and reduce their immunoreactivity.Methods To explore whether CGMP has the potential to interact with Ara h1 and Ara h2 by protein-protein molecular docking technique.Furthermore,a mixed solution of CGMP and peanut proteins(MCGP)was prepared by heating to establish a BALB/c mouse model of MCGP sensitization and peanut proteins challenge.This aimed to study the effects of MCGP on peanut allergic reactions.Additionally,the effect of interaction on the secondary structure of the two allergens was investigated by circular dichroism.Results There were secondary bonds(salt bridges,hydrogen bonds,van der Waals forces)between CGMP and Ara h1 and Ara h2,which partially act on allergenic epitopes.Compared with peanut protein allergy group,the serum levels of specific immunoglobulin E(sIgE),sIgG1,sIgG2a of Arachis hypogaea in the MCGP-sensitized group were significantly decreased;the levels of interleukin-4(IL-4),IL-5,histamine were significantly decreased;the levels of transforming growth factor-β(TGF-β)and interferon-gamma(IFN-γ)were significantly increased,and the ratio ofα-helix toβ-folding of Ara h2 in MCGP was altered.Conclusion CGMP has the ability to modify the structure of Ara h 2,combine peanut allergens epitopes,inhibit sIgE and sIgG binding to both Ara h 1 and Ara h 2,and reduce the immunoreactivity of some peanut allergens.

关 键 词：酪蛋白糖巨肽 花生 过敏原 免疫反应性 蛋白质相互作用 分子对接 

分 类 号：TS201.6[轻工技术与工程—食品科学]