Towards overcoming obstacles of type Ⅱ photodynamic therapy:Endogenous production of light,photosensitizer,and oxygen  

作　　者：Lin Yu Zhen Liu Wei Xu Kai Jin Jinliang Liu Xiaohui Zhu Yong Zhang Yihan Wu 

机构地区：[1]Department of Chemical and Environmental Engineering,Shanghai University,Shanghai 200433,China [2]School of Medicine,Shanghai University,Shanghai 200433,China [3]Department of Biomedical Engineering,City University of Hong Kong,Hong Kong,China

出　　处：《Acta Pharmaceutica Sinica B》2024年第3期1111-1131,共21页药学学报（英文版）

基　　金：supported by the National Natural Science Foundation of China(32000036)。

摘　　要：Conventional photodynamic therapy(PDT)approaches face challenges including limited light penetration,low uptake of photosensitizers by tumors,and lack of oxygen in tumor microenvironments.One promising solution is to internally generate light,photosensitizers,and oxygen.This can be accomplished through endogenous production,such as using bioluminescence as an endogenous light source,synthesizing genetically encodable photosensitizers in situ,and modifying cells genetically to express catalase enzymes.Furthermore,these strategies have been reinforced by the recent rapid advancements in synthetic biology.In this review,we summarize and discuss the approaches to overcome PDT obstacles by means of endogenous production of excitation light,photosensitizers,and oxygen.We envision that as synthetic biology advances,genetically engineered cells could act as precise and targeted“living factories”to produce PDT components,leading to enhanced performance of PDT.

关 键 词：Photodynamic therapy Cancer BIOGENESIS BIOSYNTHESIS Engineered bacteria PHOTOTHERAPY PHOTOSENSITIZERS CATALASE 

分 类 号：R730.5[医药卫生—肿瘤] TQ421.7[医药卫生—临床医学]