侧流层析技术在大肠杆菌O157:H7检测中的应用  

Review on the application of lateral flow chromatographic assay in Escherichia coli O157:H7 detection

作　　者：田歌 蒋亦佳 张赟[1] TIAN Ge;JIANG Yijia;ZHANG Yun(School of Medical Technology,Xinxiang Medical University,He’nan Xinxiang 453000,China)

机构地区：[1]新乡医学院医学技术学院,河南新乡453000

出　　处：《中国食品卫生杂志》2024年第1期107-112,共6页Chinese Journal of Food Hygiene

基　　金：河南省科技攻关项目(222102310310)。

摘　　要：大肠杆菌O157:H7作为一种常见的食源性致病菌,在低感染剂量下即可导致人类患严重疾病。侧流层析技术(LFCA)由于其具有高效分离的特性,能够满足食品中大肠杆菌O157:H7的快速检测需求。然而,目前广泛应用的LFCA方法,信号强度较弱,检测灵敏度较低,难以实现样本中低浓度大肠杆菌O157:H7的检出。因此,本文重点整理了近年来出现的新型侧流层析技术,围绕检测效率、灵敏度进行了系统性归纳,比较各方法的优势与短板,为大肠杆菌O157:H7侧流层析检测技术的发展提供重要结论性指导。As a common foodborne pathogen,Escherichia coli(E.coli)O157:H7 can cause serious diseases in human body at low infectious doses.Lateral flow chromatographic assay(LFCA)can meet the requirement of detecting E.coli O157:H7 in food samples rapidly due to its efficient separation property.At present,the widely-used LFCA methods generate weak signal strength and possess low detection sensitivity,which can hardly detect low concentration of E.coli O157:H7.Therefore,this review focuses on the newly published LFCA literature,and a systematic induction is performed concerning the detection efficiency and sensitivity of the above studies.The advantages and disadvantages of each method are compared in this review,which will provide important conclusive guidance for the development of LFCA in E.coli O157:H7 detection.

关 键 词：侧流层析技术 食源性致病菌 大肠杆菌O157:H7 检测 食品安全 

分 类 号：R155[医药卫生—营养与食品卫生学]