白茶蛋白酶解物的抗氧化与α-葡萄糖苷酶抑制力研究  

Antioxidative andα-Glucosidase Inhibition Activities of Protein Hydrolysates of White Tea

作　　者：卢学慧 陈巧玲 王弛 赵峰 LU Xue-hui;CHEN Qiao-ling;WANG Chi;ZHAO Feng(College of Pharmacy,Fujian University of Traditional Chinese Medicine,Fuzhou,Fujian 350122)

机构地区：[1]福建中医药大学药学院,福建福州350122

出　　处：《茶叶学报》2024年第1期53-61,共9页Acta Tea Sinica

基　　金：福建中医药大学引进人才科研启动项目(2801/701190097)。

摘　　要：【目的】提取和酶解制备白茶蛋白生物活性肽,并探讨其抗氧化能力和α-葡萄糖苷酶抑制率,为白茶综合利用提供理论依据。【方法】以寿眉白茶为原料,采用碱提酸沉法获得白茶蛋白粗提物,再分别以碱性蛋白酶、木瓜蛋白酶、中性蛋白酶和复配酶进行酶解,比较各酶解物的DPPH自由基清除率、ABTS自由基清除率、总还原力和α-葡萄糖苷酶抑制率差异。【结果】采用碱提(0.3 mol·L^(-1)NaOH)酸沉(pH=3.0)法获得白茶蛋白粗提物,其纯度46.6%,得率7.1%。酶解物的活性评价结果显示,木瓜蛋白酶酶解物浓度为200μg·mL^(-1)时对DPPH自由基清除率最大,为86.8%;4种蛋白酶酶解物对ABTS自由基清除能力相当;复配酶酶解物浓度为1000μg·mL^(-1)时总还原力最大,为0.930;中性蛋白酶酶解物浓度为1000μg·mL^(-1)时对α-葡萄糖苷酶抑制力最强,为97.8%。【结论】使用中性蛋白酶、复配酶和木瓜蛋白酶,有望开发具备抗氧化能力与α-葡萄糖苷酶抑制力的白茶蛋白活性肽。【Objective】The enzymatically hydrolyzed protein from white tea was analyzed for antioxidant andα-glucosidase inhibition for the development of a functional ingredient.【Method】Protein extracted by alkali solution from Shoumei white tea was acid-precipitated prior to protease hydrolysis using alcalase,papain,neutrase,or protamex.Rates of DPPH and ABTS free radical scavenging as well as total reducing power andα-glucosidase inhibition of the resulting hydrolysates were determined.【Result】The extraction applied 0.3 mol·L^(-1) NaOH to solubilize protein from the white tea and precipitated at pH 3.0 to yield 7.1%of a material containing 46.6%protein for the subsequent enzymatic hydrolysis.The hydrolysate of the 200μg·mL^(-1) papain digestion exhibited the highest DPPH radical scavenging rate at 86.8%;The four protease hydrolysates were comparable in their ability to scavenge ABTS free radicals;and that of 1000μg·mL^(-1) protamex,the total reducing power was the highest,amounting to 0.930;and that of the 1000μg·mL^(-1) neutrase,the most powerfulα-glucoglycanase inhibition at 97.8%.【Conclusion】It appeared that the application of neturase,protamex,or papain to enzymatically hydrolyze the white tea protein extract could produce peptides with significant antioxidative andα-glucosidase inhibition activities rendering a potential bioactive agent as a functional ingredient.

关 键 词：白茶 酶解物 抗氧化 Α-葡萄糖苷酶 

分 类 号：S571.1[农业科学—茶叶生产加工]