液相色谱-串联质谱法测定大米中3种香兰素的含量  

Determination of Three Vanillin in Rice by Liquid Chromatography-Mass Spectrometry

作　　者：周美丽 王瑞 卢薇 王岩 ZHOU Meili;WANG Rui;LU Wei;WANG Yan(Baoji Food and Drug Inspection and Test Center,Baoji 712100)

机构地区：[1]宝鸡市食品药品检验检测中心,宝鸡721000

出　　处：《食品工业》2024年第3期323-326,共4页The Food Industry

摘　　要：建立液相色谱-质谱/质谱法测定大米中香兰素、甲基香兰素和乙基香兰素含量的方法。3种香料在5~200ng/m L范围内线性关系良好,相关系数均大于0.99;检出限分别为5,2和3μg/kg,定量限分别为15,6和9μg/kg,均低于国家标准规定值;加标水平为30,100和300μg/kg时回收率在96.4%~106.6%之间,相对标准偏差为0.8%~3.1%(n=6)。该方法提取过程简单、检测灵敏度高、重复性非常好,能有效满足大米中香兰素、甲基香兰素和乙基香兰素的快速测定。样品测定结果显示大米中存在内源性香兰素,建议相关部门及时调整相应的国家标准限量值及监管措施,以免误判。Establish a method for determining the content of vanillin,methyl vanillin,and ethyl vanillin in rice by liquid chromatography-mass spectrometry.The three spices have good linear relationships within the range of 5-200 ng/mL,with correlation coefficients greater than 0.99.The detection limits were 5 μg/kg,2 μg/kg and 3 μg/kg,respectively,with quantification limits of 15 μg/kg,6 μg/kg and 9 μg/kg,respectively,all lower than the national standard value.The recoveries were 96.4%-106.6% and the relative standard deviations were 0.8%-3.1%(n=6) when the calibration levels were 30,100 and 300 μg/kg.This method was easy to operate,highly sensitive,and had good repeatability,and could effectively meet the rapid determination of vanillin,methyl vanillin,and ethyl vanillin in rice.The sample determination results showed the presence of endogenous vanillin in rice.It was recommended that relevant departments adjust the corresponding national standard limit values and regulatory measures in a timely manner to avoid misjudgment.

关 键 词：香兰素 甲基香兰素 乙基香兰素 大米 液相色谱-串联质谱法 

分 类 号：TS210.7[轻工技术与工程—粮食、油脂及植物蛋白工程] O657.63[轻工技术与工程—食品科学与工程]