6-姜烯酚玉米醇溶蛋白纳米颗粒的构建及其生物利用度分析  

Construction and Bioavailability Analysis of 6-Shogaol-Loaded Zein Nanoparticles

作　　者：高就 吴俊杰 焦文雅 陈宇洋 祝文轩 桑亚新[1] 王向红[1] GAO Jiu;WU Junjie;JIAO Wenya;CHEN Yuyang;ZHU Wenxuan;SANG Yaxin;WANG Xianghong(College of Food Science and Technology,Hebei Agricultural University,Baoding 071000,China)

机构地区：[1]河北农业大学食品科技学院,河北保定071000

出　　处：《食品科学》2024年第9期44-50,共7页Food Science

基　　金：河北省重点研发计划项目(21327314D);河北省三期蔬菜产业技术体系创新团队项目(HBCT2023100206)。

摘　　要：为解决6-姜烯酚(6-shogaol,6S)生物利用度低的问题,通过反溶剂沉淀法构建玉米醇溶蛋白-酪蛋白酸钠纳米颗粒(zein-sodium caseinate nanoparticles,ZCP)作为6S的纳米输送载体,对其进行物化性质表征,并通过体外模拟消化、Caco-2细胞模型及大鼠药代动力学实验考察其生物利用度。利用激光粒度仪、透射电子显微镜、傅里叶变换红外光谱对所构建6S复合纳米颗粒(ZCP-6S)的粒径分布、微观形态、化学结构进行表征。结果表明,纳米颗粒呈球形,粒径较小且分布均匀,6S可能通过非共价键与玉米醇溶蛋白相互作用。体外模拟消化结果表明,ZCP-6S的制备使6S的生物可及性提高至(75.34±9.82)%。ZCP-6S显著增强了Caco-2细胞对6S的摄取及转运,处理4 h后,细胞摄取量增加了(0.36±0.06)μg/mg;Caco-2细胞模型下室中6S的质量浓度增加了(1.06±0.06)μg/m L。大鼠药代动力学研究结果表明,纳米颗粒封装后6S的相对口服生物利用度提高了2.28倍。综上,通过玉米醇溶蛋白纳米颗粒输送载体的构建,使6S的生物可及性、细胞吸收和口服生物利用度均得到了有效提高。In order to solve the problem of the low bioavailability of 6-shogaol(6S),in this study,zein-sodium caseinate nanoparticles(ZCP)were constructed by the anti-solvent precipitation method as a nano-delivery carrier for 6S.The physicochemical properties of 6S-loaded zein nanoparticles(ZCP-6S)were characterized and the bioavailability was investigated by in vitro simulated digestion,Caco-2 cell model and pharmacokinetic studies in rats.The particle size distribution,microstructure and chemical structure of ZCP-6S were characterized by laser particle size analyzer,transmission electron microscopy(TEM)and Fourier transform infrared(FTIR)spectroscopy.The results indicated that the nanoparticles were spherical in shape,with small particle size and uniform distribution.6S might interact with zein through non-covalent bonds.The in vitro simulated digestion results showed that ZCP-6S increased the bioaccessibility of 6S to(75.34±9.82)%.Moreover,ZCP-6S significantly enhanced the uptake and transport of 6S by Caco-2 cells.After 4 h treatment,the cell uptake increased by(0.36±0.06)μg/mg.The concentration of 6S in the basolateral side of the Caco-2 cell model increased by(1.06±0.06)μg/mL.In addition,pharmacokinetic studies showed that the relative oral bioavailability of 6S increased by 3.28 times after nanoparticle encapsulation.In summary,the bioaccessibility,cellular absorption and oral bioavailability of 6S were effectively improved by its encapsulation into zein nanoparticles.

关 键 词：6-姜烯酚 玉米醇溶蛋白 酪蛋白酸钠 纳米颗粒 生物利用度 

分 类 号：TS202.1[轻工技术与工程—食品科学]