杏干多酚的提取工艺及体外抗氧化活性研究  

Study on Extraction Technology and in Vitro Antioxidant Activity of Dried Apricot Polyphenols

作　　者：赵彩云 高文静 吴斌 郑力 傅樱花 ZHAO Caiyun;GAO Wenjing;WU Bin;ZHENG Li;FU Yinghua(Xinjiang Key Laboratory of Biological Resources and Genetic Engineering,College of Life Science and Technology,Xinjiang University,Urumqi 830017,China;Institute of Farm Product Storage and Processing,Xinjiang Academy of Agricultural Sciences,Urumqi 830002,China)

机构地区：[1]新疆大学生命科学与技术学院,新疆生物资源基因工程重点实验室,新疆乌鲁木齐830017 [2]新疆农业科学院农产品贮藏加工研究所,新疆乌鲁木齐830002

出　　处：《保鲜与加工》2024年第5期61-68,共8页Storage and Process

基　　金：新疆维吾尔自治区重点研发任务专项计划项目(2022B03006-2)。

摘　　要：新疆地区杏资源丰富,杏含有多酚类物质等多种生物活性成分,研究杏干多酚的提取工艺可为进一步利用杏资源开发健康产品奠定基础。以新疆赛买提杏干为原料,采用水浴浸提法提取杏干多酚,并采用响应面试验优化杏干多酚提取工艺,同时考察杏干多酚体外抗氧化活性。结果表明,杏干多酚提取最佳工艺为:乙醇溶液体积分数70%,液料比21∶1(mL/g),浸提时间2.6 h,浸提温度51℃,在此条件下杏干多酚提取量为(4.1189±0.0201)mg/g;各因素对杏干多酚提取量的影响大小顺序为:液料比>乙醇体积分数>浸提温度>浸提时间;抗氧化试验结果表明,杏干多酚对1,1-二苯基-2-三硝基苯肼(DPPH)自由基和羟基自由基的清除能力较强,对2,2-联氨-双-3-乙基苯并噻唑啉-6-磺酸(ABTS)阳离子自由基和超氧阴离子表现出一定的清除能力。最佳提取工艺制备的杏干多酚具有一定的体外抗氧化活性。Apricots are abundant in Xinjiang region,and contain a variety of bioactive components such as polyphe⁃nols.Investigation on the extraction process of dried apricot polyphenols can lay a foundation for further exploiting apricot resources to develop healthy products.In this paper,Saimaiti dried apricots of Xinjiang were used as raw mate⁃rials,and the water immersion was employed for polyphenols extraction.The extraction process was optimized by re⁃sponse surface experiment,and the in vitro antioxidant activity of polyphenols was investigated.The results showed that the optimal extraction process was determined as follows:ethanol solution volume fraction of 70%,liquid-solid ra⁃tio of 21∶1(mL/g),extraction time of 2.6 h,extraction temperature of 51℃.Under these conditions,the amount of poly⁃phenols extracted from dried apricot was(4.1189±0.0201)mg/g.The main order of influencing factors on the contents of apricot polyphenols was identified as follows:liquid-solid ratio>ethanol volume fraction>extraction temperature>extraction time.The antioxidant test results showed that dried apricot polyphenols presented strong abilities to scav⁃enge DPPH radicals and hydroxyl radicals,and showed certain capacities to scavenge ABTS cation radicals and super⁃oxide anions,indicating that dried apricot polyphenols had a certain antioxidant activity in vitro.

关 键 词：杏干 多酚提取 工艺 抗氧化活性 

分 类 号：TS201.4[轻工技术与工程—食品科学]