Additive neurorestorative effects of exercise and docosahexaenoic acid intake in a mouse model of Parkinson’s disease  

作　　者：Olivier Kerdiles Méryl-Farelle Oye Mintsa Mi-mba Katherine Coulombe Cyntia Tremblay VincentÉmond Martine Saint-Pierre Clémence Rouxel Line Berthiaume Pierre Julien Francesca Cicchetti Frédéric Calon 

机构地区：[1]Facultéde pharmacie,UniversitéLaval,1050 Avenue de la Médecine,Quebec,QC,Canada [2]Axe Neuroscience,Centre de recherche du CHU de Québec-UniversitéLaval(Pavillon CHUL),2705 Boulevard Laurier,Quebec,QC,Canada [3]Institute of Nutrition and Functional Foods,Quebec,QC,Canada [4]Optinutribrain International Associated Laboratory(NutriNeuro,France,INAF,Canada),Quebec,QC,Canada [5]Axe Endocrinologie et Néphrologie,Centre de recherche du CHU de Québec-UniversitéLaval,Quebec,QC,Canada [6]Département de Médecine,Facultéde Médecine,UniversitéLaval,Quebec,QC,Canada [7]Département de Psychiatrie et Neurosciences,Facultéde Médecine,Quebec,QC,Canada

出　　处：《Neural Regeneration Research》2025年第2期574-586,共13页中国神经再生研究（英文版）

基　　金：supported by funding from Parkinson Canada;funded by a scholarship from Parkinson Canada;a scholarship from Fonds d’Enseignement et de Recherche (FER) (Faculty of Pharmacy, Université Laval)

摘　　要：There is a need to develop interventions to slow or reverse the degeneration of dopamine neurons in Parkinson’s disease after diagnosis.Given that preclinical and clinical studies suggest benefits of dietary n-3 polyunsaturated fatty acids,such as docosahexaenoic acid,and exercise in Parkinson’s disease,we investigated whether both could synergistically interact to induce recovery of the dopaminergic pathway.First,mice received a unilateral stereotactic injection of 6-hydroxydopamine into the striatum to establish an animal model of nigrostriatal denervation.Four weeks after lesion,animals were fed a docosahexaenoic acid-enriched or a control diet for the next 8 weeks.During this period,the animals had access to a running wheel,which they could use or not.Docosahexaenoic acid treatment,voluntary exercise,or the combination of both had no effect on(i)distance traveled in the open field test,(ii)the percentage of contraversive rotations in the apomorphine-induction test or(iii)the number of tyrosine-hydroxylase-positive cells in the substantia nigra pars compacta.However,the docosahexaenoic acid diet increased the number of tyrosine-hydroxylase-positive terminals and induced a rise in dopamine concentrations in the lesioned striatum.Compared to docosahexaenoic acid treatment or exercise alone,the combination of docosahexaenoic acid and exercise(i)improved forelimb balance in the stepping test,(ii)decreased the striatal DOPAC/dopamine ratio and(iii)led to increased dopamine transporter levels in the lesioned striatum.The present results suggest that the combination of exercise and docosahexaenoic acid may act synergistically in the striatum of mice with a unilateral lesion of the dopaminergic system and provide support for clinical trials combining nutrition and physical exercise in the treatment of Parkinson’s disease.

关 键 词：6-HYDROXYDOPAMINE DOPAMINE dopamine transporter EXERCISE neurorestoration Parkinson’s disease polyunsaturated fatty acids omega-3 

分 类 号：R742.5[医药卫生—神经病学与精神病学] R-332[医药卫生—临床医学]