基于响应面法优化酒糟酶解工艺  

Optimization of enzymatic hydrolysis process of distiller's grains based on response surface method

作　　者：庞琼 薛莎莎 李卓渊 赵佳 郝建伟 史胜利 刘晓夏 罗爱国 PANG Qiong;XUE Shasha;LI Zhuoyuan;ZHAO Jia;HAO Jianwei;SHI Shengli;LIU Xiaoxia;LUO Aiguo(College of Food Science and Engineering,Shanxi Agricultural University,Jinzhong 030801,China;Department of Biological Science and Technology,Jinzhong University,Jinzhong 030619,China;Shanxi Compound Condiment Technology Innovation Center,Jinzhong 030619,China)

机构地区：[1]山西农业大学食品科学与工程学院,山西晋中030801 [2]晋中学院生物科学与技术系,山西晋中030619 [3]山西省复合调味品技术创新中心,山西晋中030619

出　　处：《中国酿造》2024年第5期135-143,共9页China Brewing

基　　金：山西省2021年度留学人员科技活动择优资助项目(20210050);山西省高等学校科技创新计划创新平台项目(2022P022);晋中学院“1331工程”酿造副产物资源高值化利用协同创新中心项目(jzxyxtcxzx202104)。

摘　　要：为提高酒糟纤维素酶解效率,促进酒糟资源化利用,该试验以酿造副产物酒糟为原料,以还原糖得率为评价指标,结合单因素试验结果、Plackett-Burman试验筛选显著因素和响应面法优化酒糟酶解工艺条件,并采用扫描电镜(SEM)观察酒糟原料、芬顿试剂预处理酒糟、酶解酒糟的显微结构。结果表明,最佳酶解工艺条件为:处理时间27 h、处理pH 4.7、纤维素酶添加量1060 U/g、酶解pH 4.9。在此优化条件下,还原糖得率为64.44%,较对照组提高148.37%。SEM结果显示,酒糟经过芬顿试剂预处理和酶解后表面形态呈现蓬松状态,木质纤维结构被破坏,孔隙率增加;进一步酶解后块状物质明显减少,露出许多小孔,表明酒糟还原糖得率与表面微观形貌变化直接相关。In order to improve the enzymatic hydrolysis efficiency of distiller's grains and promote the resource utilization of distiller's grains,using distiller's grains as the raw material and the yield of reducing sugar as the evaluation index,combined with the single factor test results,the significant factors were screened by Plackett-Burman tests,and the enzymatic hydrolysis process conditions of distiller's grains were optimized by response surface method.The microstructure of raw material,Fenton reagent pretreated and enzymatic hydrolyzed distiller's grains were observed by scanning electron microscope(SEM).The results showed that the optimum enzymatic conditions were as follows:treatment time 27 h,pH 4.7,cellulase addition 1060 U/g,enzymatic hydrolysis pH 4.9.Under the optimized conditions,the yield of reducing sugar was 64.44%,which was 148.37%higher than that of the control group.SEM results showed that after Fenton reagent pretreatment and enzymolysis,the surface morphology of distiller's grains was bulky,the structure of wood fiber was destroyed,and the porosity increased.After further enzymatic hydrolysis,the bulk material was significantly reduced and many small holes were exposed,which indicated that the yield of reducing sugar was directly related to the change of surface morphology.

关 键 词：酒糟 芬顿试剂 预处理 酶解工艺 响应面优化 

分 类 号：TS261.9[轻工技术与工程—发酵工程]