不同接种量下不同浓度虾壳粉对红曲霉发酵产红曲色素对比研究  

Comparative Study on Monascus Pigment Produced by Monascus Fermentation with Different Concentrations of Shrimp Shell Powder with Different Inoculation Amounts

作　　者：刘枣 陈鑫 李娇 王伟平 LIU Zao;CHEN Xin;LI Jiao;WANG Wei-ping(School of Biological Engineering and Food Science,Hubei University of Technology,Wuhan 430068,China)

机构地区：[1]湖北工业大学生物工程与食品学院,武汉430068

出　　处：《中国食物与营养》2024年第5期43-46,51,共5页Food and Nutrition in China

基　　金：国家自然科学基金项目(项目编号:31101349)。

摘　　要：目的:小龙虾虾壳废弃物排放造成环境污染和资源浪费,红曲霉可以转化利用虾壳蛋白质,达到对红曲色素生产的工艺优化。方法:以小龙虾虾壳废弃物蛋白质为唯一氮源,利用红曲霉菌种MonascusGN01进行液态发酵,探究不同接种量对红曲霉液态发酵产红曲色素和桔霉素的影响,得到低桔霉素高红曲色素生产工艺。结果:在5%接种量下,虾壳粉添加量为0.5%的发酵液红曲红色素的色价最高,为10U/mL,其桔霉素含量为0.004mg/L;在10%接种量下,虾壳粉添加量在1.1%时的发酵液红曲红色素色价最高,为49.75 U/mL,其桔霉素含量为0.032mg/L。结论:在实际生产中应根据不同的目的选用合适的接种量,为红曲霉发酵虾壳确定初步的工艺参数。【Objective】The discharge of crawfish shell waste causes environmental pollution and resource waste.In order to optimize the production process of Monascus pigment,Monascus can transform and utilize the protein of crawfish shell.【Method】The protein from crawfish shell waste was used as the sole nitrogen source to conduct liquid fermentation with Monascus GN01.The effects of different inoculum sizes on Monascus and citrinin production by Monascus were investigated,and the production process of low citrinin and high Monascus pigment was obtained【.Result】The fermentation broth containing 0.5%shrimp shell powder had the highest color value of 10 U/mL and the content of citrinin was 0.004 mg/L under the condition of 5%inoculum.When the amount of shrimp shell powder was 1.1%,the fermentation broth had the highest monyeast red pigment value of 49.75 U/mL under 10%inoculum.The content of citrinin was 0.032 mg/L.【Conclusion】In actual production,the appropriate inoculation amount should be selected according to different purposes,and the preliminary technological parameters of crawfish shell fermentation by Monascus were determined.

关 键 词：红曲霉 虾壳 液态发酵 红曲色素 接种量 

分 类 号：TS254.9[轻工技术与工程—水产品加工及贮藏工程]