响应面法优化硒化木寡糖的制备工艺及其抗氧化活性分析  

Optimization of preparation process of selenized xylo-oligosaccharides by response surface methodology and analysis of its antioxidant activity

作　　者：王清[1] 陈晖 刘涛[1] 王如江 郑雪珂 桑大席 WANG Qing;CHEN Hui;LIU Tao;WANG Ru-jiang;ZHENG Xue-ke;SANG Da-xi(College of Food Science and Engineering,Xinyang Agriculture and Forestry University,Xinyang 464000,Henan,China)

机构地区：[1]信阳农林学院食品科学与工程学院,河南信阳464000

出　　处：《粮食与油脂》2024年第6期76-82,共7页Cereals & Oils

基　　金：河南省科技攻关项目(232102230071);河南省高等学校重点科研项目(23B550006、24B180016);信阳农林学院高水平科研孵化器建设基金(FCL202110);信阳农林学院青年骨干教师培养项目;信阳农林学院青年基金项目(QN2023032)。

摘　　要：以木寡糖(XOS)为原料,Na_(2)SeO_(3)为硒化剂,BaCl_(2)为催化剂制备硒化木寡糖(XOS-Se)。以XOS-Se中Se含量为评价指标,采用单因素试验及响应面试验优化XOS-Se的制备工艺。通过紫外光谱(UV)及傅里叶红外光谱(FT-IR)扫描确定XOS是否硒化成功。通过DPPH自由基、ABTS自由基以及羟基自由基的清除率综合评价XOS-Se的抗氧化活性。结果表明:最佳工艺条件为Na_(2)SeO_(3)与XOS质量比0.67∶1、反应时间8.7 h、BaCl_(2)添加量1.62 g,在此条件下制备的XOS-Se中Se含量可达3.124 mg/g。UV及FT-IR扫描表明XOS被硒化成功。抗氧化活性试验显示,XOS-Se的抗氧化活性总体上显著高于XOS和Na_(2)SeO_(3)(P<0.05),表明硒化修饰可较好地提高XOS的抗氧化活性。Selenized xylo-oligosaccharides(XOS-Se)were prepared using xylo-oligosaccharides(XOS)as raw materials,Na_(2)SeO_(3)as selenide,and BaCl_(2)as catalyst.Taking the selenium content in XOS-Se as the evaluation index,the preparation process of XOS-Se was optimized by single factor experiment and response surface experiment.Whether XOS had been successfully selenized was determined by UV scanning and Fourier-transform infrared spectroscopy(FT-IR).The antioxidant activity of XOS-Se was comprehensively evaluated by measuring its ability to scavenge DPPH free radicals,ABTS free radicals and hydroxyl radicals.The results showed that the optimal process conditions were mass ratio of Na_(2)SeO_(3)to XOS 0.67:1,reaction time 8.7 h and BaCl_(2)dosage 1.62 g.Under the conditions,the selenium content in XOS-Se reached 3.124 mg/g.The results of UV and FT-IR scanning showed that XOS had been successfully selenized.The antioxidant activity test showed that the overall antioxidant activity of XOS-Se was significantly higher than that of XOS and Na_(2)SeO_(3)(P<0.05),indicating that selenization modification could improve the antioxidant properties of XOS.

关 键 词：木寡糖 响应面法 制备工艺 抗氧化活性 

分 类 号：TS201.1[轻工技术与工程—食品科学]