盐渍凡纳滨对虾抗菌肽PV-M7对副溶血性弧菌的抑菌活性  

Antibacterial Activity of Salted Penaeus vannamei Antimicrobial Peptide PV-M7 against Vibrio parahaemolyticus

作　　者：戴静怡 金日天 林蓉 翁武银[1,2,3] 邓尚贵 杨燊 Dai Jingyi;Jin Ritian;Lin Rong;Weng Wuyin;Deng Shanggui;Yang Shen(College of Ocean Food and Biological Engineering,Jimei University,Xiamen 361021,Fujian;Fujian Provincial Key Laboratory of Food Microbiology and Enzyme Engineering,Xiamen 361021,Fujian;Collaborative Innovation Center of Seafood Deep Processing,Dalian Polytechnic University,Dalian 116034,Liaoning;Zhejiang Xingye Group Co.,Ltd.,Zhoushan 316014,Zhejiang)

机构地区：[1]集美大学海洋食品与生物工程学院,福建厦门361021 [2]福建省食品微生物与酶工程重点实验室,福建厦门361021 [3]大连工业大学,海洋食品精深加工关键技术省部共建协同创新中心,辽宁大连116034 [4]浙江兴业集团,浙江舟山316014

出　　处：《中国食品学报》2024年第5期182-189,共8页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家重点研发计划项目(2020YFD09009)。

摘　　要：抗菌肽是食品工业的新型生物防腐剂,而盐渍发酵食品是抗菌肽的来源之一。采用超高效液相色谱-质谱联用技术从盐渍凡纳滨对虾中鉴定小分子多肽序列,利用生物信息学分析筛选潜在的抗菌肽。通过最低抑菌浓度、时间-杀灭曲线、细胞膜通透性和圆二色谱等试验评价抗菌肽对副溶血性弧菌的抑菌活性及机制。结果表明:盐渍凡纳滨对虾中一种新型抗菌肽(PV-M7)对副溶血性弧菌的最低抑菌质量浓度为15.6μg/mL,其在1 h内即可产生很好的抑菌作用。PV-M7作用于副溶血性弧菌后,可使细胞膜通透性增加,胞内大分子物质(核酸、蛋白质)泄露,同时,细胞膜表面模糊,细胞质密度减小。当PV-M7接触细胞膜时,它的二级结构由无规则卷曲转变成α-螺旋,这种结构转换是其发挥抑菌活性的关键作用机制。Antimicrobial peptides are novel biological preservatives in the food industry,and salted fermented foods are one of the sources of antimicrobial peptides(AMPs).In this study,ultra-performance liquid chromatography-mass spectrometry(UPLC-MS)was used to identify small molecule peptide sequences from salted Penaeus vannamei,and potential antimicrobial peptides were analyzed and screened by bioinformatics.Antibacterial activity and mechanism of AMPs against Vibrio parahaemolyticus were evaluated by minimum inhibitory concentration,time-kill curve,cell membrane permeability,and circular dichroism.The results showed that the minimum inhibitory concentration of the novel AMP(PV-M7)from salted Penaeus vannamei was 15.6μg/mL against Vibrio parahaemolyticus,and the antibacterial activity was exerted within 1 h.After PV-M7 acted on Vibrio parahaemolyticus,it could increase the permeability of the cell membrane,leak the macromolecular substances(nucleic acid,protein)in the cell,at the same time,the surface of the cell membrane was blurred,and the density of the cytoplasm decreased.When PV-M7 contacts the cell membrane,its secondary structure changed from random coil toα-helical,this structural transformation was the key mechanism of its antibacterial activity.

关 键 词：凡纳滨对虾 副溶血性弧菌 抗菌肽 结构转换 抑菌机制 

分 类 号：TS254.4[轻工技术与工程—水产品加工及贮藏工程]