泥鳅抗氧化活性肽的制备、鉴定及其生物活性  

Preparation,Identification and Activity of Antioxidant Active Peptides from Misgurnus anguillicaudatus

作　　者：窦宝杰 翁南海 卢静 郭全友[3] 吕明生 王淑军 Dou Baojie;Weng Nanhai;Lu Jing;Guo Quanyou;LüMingsheng;Wang Shujun(Jiangsu Provincial Key Laboratory of Marine Biological Resources and Environment,Jiangsu Ocean University,Lianyungang 222005,Jiangsu;Key Laboratory of Marine Biotechnology of Jiangsu Province,Jiangsu Ocean University,Lianyungang 222005,Jiangsu;East China Sea Fishery Research Institute,Chinese Academy of Fishery Sciences,Shanghai 200090)

机构地区：[1]江苏海洋大学江苏省海洋生物资源与环境重点实验室,江苏连云港222005 [2]江苏海洋大学江苏省海洋生物技术重点实验室,江苏连云港222005 [3]中国水产科学院东海水产研究所,上海200090

出　　处：《中国食品学报》2024年第5期223-232,共10页Journal of Chinese Institute Of Food Science and Technology

基　　金：江苏省连云港市521项目(LYG06521202107);江苏省海洋资源与环境重点实验室基金项目(SH20201204)。

摘　　要：泥鳅是亚洲常见的淡水鱼,具有较高的营养和药用价值。本研究采用短小芽孢杆菌4SP5分泌的蛋白酶水解泥鳅肉糜,超滤获得不同分子质量的水解产物。其中,分子质量小于5 ku的组分的抗氧化活性最强。经G25色谱过滤纯化,LC-MS/MS测序和鉴定过滤组分,获得6种潜在的生物活性肽。其中寡肽AFRVPTP具有良好的DPPH自由基清除活性(IC5037μg/mL)、羟自由基清除活性、超氧阴离子清除活性(IC500.73 mg/mL)。此外,该寡肽还具有抑制α-葡萄糖苷酶的作用,IC50为15.86 mg/mL。经在线预测,该寡肽无毒性和致敏性。分子对接结果表明,AFRVPTP通过与α-葡萄糖苷酶催化域的关键氨基酸结合而发挥作用,其可与Keap1活性口袋中的关键氨基酸残基(Asn387、Arg415、Tyr334、Arg380、Gln530、Tyr525、Arg483和Ser508)相互作用,表明该寡肽具有调节体内抗氧化作用的潜力。研究结果为泥鳅的精深加工提供参考依据。Misgurnus anguillicaudatus(loach)is a common freshwater fish in Asia,which has higher nutritional and medical value.In this study,the loach was hydrolyzed with protease secreted by Bacillus pumilus 4SP5 and the hydrolysis products of different molecular weights were obtained by ultrafiltration.Among them,the fractions smaller than 5 ku showed the strongest antioxidant activity.The filtration components were purified by G25 chromatography filtration and sequenced and identified by LC-MS/MS,and 6 potential bioactive peptides were obtained.Among them,the oligopeptide AFRVPTP had good DPPH scavenging activity(IC5037μg/mL),hydroxyl radical scavenging activity,and superoxide anion scavenging activity(IC500.73 mg/mL).The oligopeptide was also shown to inhibitα-glucosidase with an IC50 of 15.86 mg/mL and was predicted to be non-toxic and non-sensitizing online.The results of molecular docking showed that AFRVPTP acted by binding key amino acids in the catalytic domain of the enzyme.It could interact with key amino acids residue of the active domain of Keap1,which indicated that the AFRVPTP had the application potential to regulate the antioxidation(Asn387,Arg415,Tyr334,Arg380,Gln530,Tyr525,Arg483,Ser508)in vivo.The results provided experimental basis for the intensive processing of loach.

关 键 词：泥鳅 生物活性肽 抗氧化 α-葡萄糖苷酶抑制 分子对接 

分 类 号：TS254.4[轻工技术与工程—水产品加工及贮藏工程]