Sinomenine increases osteogenesis in mice with ovariectomyinduced bone loss by modulating autophagy  

作　　者：Hai-Xiang Xiao Lei Yu Yu Xia Kai Chen Wen-Ming Li Gao-Ran Ge Wei Zhang Qing Zhang Hong-Tao Zhang De-Chun Geng 

机构地区：[1]Department of Orthopedics,The Fourth Affiliated Hospital of Soochow University,Suzhou Dushu Lake Hospital,Medical Centre of Soochow University,Suzhou 215006,Jiangsu Province,China [2]Department of Orthopedics,Jingjiang People’s Hospital Affiliated to Yangzhou University,Jingjiang 214500,Jiangsu Province,China [3]Department of Orthopedics,The First Affiliated Hospital of Soochow University,Suzhou 215006,Jiangsu Province,China [4]Department of Orthopedics,Hai’an People’s Hospital,Hai’an 226600,Jiangsu Province,China [5]Department of Orthopedics,The Affiliated Huai’an Hospital of Xuzhou Medical University,Huai’an Second People’s Hospital,Xuzhou 223002,Jiangsu Province,China [6]Department of Orthopaedics,The First Affiliated Hospital of Soochow University,Suzhou 215006,Jiangsu Province,China

出　　处：《World Journal of Stem Cells》2024年第5期486-498,共13页世界干细胞杂志（英文版）（电子版）

基　　金：Supported by National Natural Science Foundation of China,No.82072425.

摘　　要：BACKGROUND A decreased autophagic capacity of bone marrow mesenchymal stromal cells(BMSCs)has been suggested to be an important cause of decreased osteogenic differentiation.A pharmacological increase in autophagy of BMSCs is a potential therapeutic option to increase osteoblast viability and ameliorate osteoporosis.AIM To explore the effects of sinomenine(SIN)on the osteogenic differentiation of BMSCs and the underlying mechanisms.METHODS For in vitro experiments,BMSCs were extracted from sham-treated mice and ovariectomized mice,and the levels of autophagy markers and osteogenic differentiation were examined after treatment with the appropriate concen-trations of SIN and the autophagy inhibitor 3-methyladenine.In vivo,the therapeutic effect of SIN was verified by establishing an ovariectomy-induced mouse model and by morphological and histological assays of the mouse femur.RESULTS SIN reduced the levels of AKT and mammalian target of the rapamycin(mTOR)phosphorylation in the phosphatidylinositol 3-kinase(PI3K)/AKT/mTOR signaling pathway,inhibited mTOR activity,and increased autophagy ability of BMSCs,thereby promoting the osteogenic differentiation of BMSCs and effectively alleviating bone loss in ovariectomized mice in vivo.CONCLUSION The Chinese medicine SIN has potential for the treatment of various types of osteoporosis,bone homeostasis disorders,and autophagy-related diseases.

关 键 词：SINOMENINE OSTEOGENESIS AUTOPHAGY OVARIECTOMY OSTEOPOROSIS 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]