低共熔溶剂提取虾壳中虾青素工艺优化及机理初探  

Optimization of process and mechanism of extracting astaxanthin from shrimp shells with deep eutectic solvent

作　　者：刘宇航 相欢 黄卉[2] 魏涯[2] 岑剑伟[2] 潘创 李春生[2] 赵永强[2] 郝淑贤[2] LIU Yuhang;XIANG Huan;HUANG Hui;WEI Ya;CEN Jianwei;PAN Chuang;LI Chunsheng;ZHAO Yongqiang;HAO Shuxian(College of Food Science and Technology,Shanghai Ocean University,Shanghai 201306,China;South China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences/Key Laboratory of Aquatic Product Processing,Ministry of Agriculture and Rural Affairs/National R&D Center for Aquatic Product Processing,Guangzhou 510300,China)

机构地区：[1]上海海洋大学食品学院,上海201306 [2]中国水产科学研究院南海水产研究所/农业农村部水产品加工重点实验室/国家水产品加工技术研发中心,广东广州510300

出　　处：《南方水产科学》2024年第3期164-172,共9页South China Fisheries Science

基　　金：广东省重点领域研发计划项目(2021B0202060002);中国水产科学研究院中央级公益性科研院所基本科研业务费专项资金资助(2023TD78);中国水产科学研究院南海水产研究所中央级公益性科研院所基本科研业务费专项资金资助(2021SD07)。

摘　　要：虾壳中虾青素的高效安全提取技术是促进虾类加工副产物变废为宝的途径之一。利用乳酸-氯化胆碱构成的低共熔溶剂提取虾壳中的虾青素,在单因素实验基础上,采用响应面实验优化提取工艺;通过低共熔溶剂的物化性质分析探究其对虾青素的提取机理。优化后的工艺条件为乳酸-氯化胆碱摩尔比为2.5∶1,含水量(w)为9.5%,超声时间为30.5 min,实际得率为24.41μg·g-1。傅里叶变换红外光谱分析表明低共熔溶剂合成时官能团并未发生改变,二者融合产生的氢键可能是促进虾青素高效提取的主要原因,黏度会在一定程度上影响产品的提取过程。Development of efficient and safe extraction technology of astaxanthin from shrimp shells is crucial for transform-ing waste into valuable resources.In this study,astaxanthin in shrimp shells was extracted with deep eutectic solvent composed of lactic acid-choline chloride.A single-factor test was carried out to explore the influence of extraction conditions on the extrac-tion rate,and a response surface test was adopted to optimize the extraction process.The mechanism of astaxanthin extraction with deep eutectic solvent was explored by physical and chemical analysis.The optimized process conditions were:mo-lar ratio of 2.5∶1,water content of 9.5%,ultrasonic time of 30.5 min,and actual yield of 24.41μg·g-1.The Fourier transform in-frared spectroscopy shows that there was no change in functional groups during deep eutectic solvent synthesis,but hydrogen bond might be the reason for effective extraction of astaxanthin,and viscosity would affect the extraction process of product.

关 键 词：虾青素 低共熔溶剂 乳酸 氯化胆碱 

分 类 号：TS201.2[轻工技术与工程—食品科学]