低温等离子体预处理对超声辅助提取姜粉总黄酮的影响及机制初探  

Effects and Mechanisms of Low-temperature Plasma Pretreatment on the Ultrasound-assisted Extraction Process of Total Flavonoids from Ginger Powder

作　　者：徐艳阳 李雪凤 柴雨行 陈云洁 王雪松[2] XU Yanyang;LI Xuefeng;CHAI Yuxing;CHEN Yunjie;WANG Xuesong(College of Food Science and Engineering,Jilin University,Changchun 130062,China;College of Life Science,Jilin University,Changchun 130000,China)

机构地区：[1]吉林大学食品科学与工程学院,吉林长春130062 [2]吉林大学生命科学学院,吉林长春130000

出　　处：《食品工业科技》2024年第13期159-167,共9页Science and Technology of Food Industry

基　　金：吉林省科技发展计划项目(20200708055YY);吉林省科技发展计划项目(0200402064NC)。

摘　　要：目的:探究低温等离子体预处理对超声辅助提取姜粉总黄酮的提取效果,并对其可能机制进行初步探讨,为姜粉总黄酮的提取提供一种新方法。方法:以姜粉中总黄酮得率为考察指标,以低温等离子体放电电源功率、处理时间、进气量为考察因素,进行单因素实验和三因素三水平响应面优化试验。并应用高效液相色谱法测定低温等离子体处理前后姜粉中21种多酚类化合物的含量,通过扫描电镜(scan electronic microscope,SEM)和傅里叶变换红外光谱(Fourier transform infrared spectroscopy,FTIR)对低温等离子体处理前后姜粉的结构进行分析。结果:姜粉中总黄酮的最佳提取条件为:真空度小于100 Pa,放电电源功率295 W、处理时间60 s,进气量150 cm^(3)/min,总黄酮得率为26.9 mg/g。色谱结果表明21种多酚类化合物得到较好的分离,且精密度高(relative standard deviation,RSD≤3.75%)、稳定性好(RSD≤4.25%)、重复性好(RSD≤4.75%)、加标回收结果准确可靠(平均回收率84.11%~100.86%)。低温等离子体处理后姜粉总黄酮的含量增加,酚酸的含量下降;低温等离子体处理增加了姜粉中淀粉颗粒以及细胞壁碎片的表面粗糙度,并且导致木质素的分解,加大了提取溶剂与姜粉的接触面积,因此有利于溶剂的渗透。结论:超声法辅助低温等离子体处理是提取姜粉总黄酮的一种有效方法。Objective:Effects of low-temperature plasma pretreatment assisted by ultrasound on the extraction of total flavonoids from ginger powder were studied,and its mechanism was preliminary discussed,and provided a new method for the extraction of total flavonoids from ginger powder.Methods:The single-factor tests and three-factor three-level response surface optimization tests were carried out with the yield of total flavonoids in ginger powder as the index and discharge power,treatment time and air intake volume of low-temperature plasma as factors.Meanwhile,the contents of 21 kinds of polyphenols in ginger powder were determined by HPLC before and after low-temperature plasma treatment,and the structure of ginger powder were characterized by scanning electron microscope(SEM)and Fourier transform infrared spectroscopy(FTIR).Results:The optimum extraction conditions for total flavonoids from ginger powder were as follows:Vacuum degree less than 100 Pa,discharge power of 295 W,treatment time of 60 s,air intake volume of 150 cm^(3)/min,and the yield of total flavonoids was 26.9 mg/g.Chromatographic results showed that 21 kinds of polyphenols were separated with high precision(relative standard deviation,RSD≤3.75%),good stability(RSD≤4.25%),good repeatability(RSD≤4.75%)and accurate and reliable recovery rate(average recovery rate of 84.11%~100.86%).After low-temperature plasma treatment,total flavonoids content in ginger was increased,and phenolic acids content was decreased.Etching effect of low-temperature plasma increased the surface roughness of starch particles and cell wall fragments in ginger powder and led to the decomposition of lignin,which increased the contact area between solvent and ginger powder,and it was conducive to solvent penetration.Conclusion:Ultrasound-assisted low-temperature plasma treatment is an effective method to extract total flavonoids from ginger powder.

关 键 词：低温等离子体 姜粉 总黄酮含量 多酚类化合物 高效液相色谱法 

分 类 号：TS201.2[轻工技术与工程—食品科学]