鲍鱼脏器碱提多糖制备工艺及对L929细胞H_(2)O_(2)氧化损伤的修复活性  

Preparation Process of Alkali-extracted Polysaccharides from Abalone Viscera and Its Repair Activity against H_(2)O_(2)Oxidative Damage in L929 Cells

作　　者：吴启赐 林志超[1] 潘晓明 薛钰[1] 黄家福[1] 潘裕添[1] WU Qici;LIN Zhichao;PAN Xiaoming;XUE Yu;HUANG Jiafu;PAN Yutian(Engineering Technological Center of Mushroom Industry,Minnan Normal University,Zhangzhou 363000,China;Mendel(Xiamen)Biotechnology Co.,Ltd.,Xiamen 361000,China)

机构地区：[1]闽南师范大学菌物产业工程技术中心,福建漳州363000 [2]萌得尔(厦门)生物科技有限公司,福建厦门361000

出　　处：《食品工业科技》2024年第13期204-211,共8页Science and Technology of Food Industry

基　　金：福建省自然科学基金面上项目(2020J01822);福建省科技厅对外合作项目(2022I0022);国家科技项目备案(2021L3027);闽南师范大学培育项目(MSPY2022002,MSPY202101)。

摘　　要：目的:优化鲍鱼脏器碱提多糖(Alkali-extracted abalone viscera polysaccharides,Aavp)提取工艺,并研究纯化Aavp对L929细胞H_(2)O_(2)氧化损伤的预防和修复作用,以期为鲍鱼脏器碱提多糖的开发及应用提供参考。方法:通过热碱提醇沉法获得鲍鱼脏器碱提粗多糖,并利用响应面法优化提取工艺,最后分析纯化Aavp对L929细胞H_(2)O_(2)氧化损伤的预防和修复能力。结果:鲍鱼脏器碱提粗多糖最佳提取工艺条件为:料液比1:150 g/mL,提取时间2 h,提取温度70℃,在此条件下,鲍鱼脏器碱提粗多糖得率为8.57%。在L929细胞H_(2)O_(2)氧化损伤预防试验中,不同剂量的纯化Aavp(20、50和100μg/mL)对L929细胞氧化损伤均具有极显著的预防作用(P<0.001),但没有表现出剂量效应,其中中剂量组(50μg/mL)的细胞存活率最高(71.94%±3.08%),略优于阳性对照的维生素E组,但无显著性差异。在修复试验中,不同剂量的纯化Aavp(20、50和100μg/mL)对L929细胞氧化损伤均具有极显著的修复效果(P<0.001),并呈现剂量效应,而高剂量组(100μg/mL)的细胞存活率最高(90.93%±1.17%),略高于阳性对照的维生素E组(87.96%±3.05%)。结果表明鲍鱼脏器碱提多糖对L929细胞H_(2)O_(2)氧化损伤具有出较好的预防和修复作用。结论:鲍鱼脏器碱提多糖具有一定的L929细胞H_(2)O_(2)氧化损伤修复能力。Objective:The extraction process of alkali-extracted abalone viscera polysaccharides(Aavp)was optimized,and the preventive and repair effects of purified Aavp on H_(2)O_(2)oxidative damage on L929 cells were studied to provide a reference for the development and application of Aavps.Methods:Crude Aavp was obtained by hot alkali extraction followed by alcohol precipitation,and its extraction process was optimized by the response surface method.The preventive and repair abilities of H_(2)O_(2)oxidative damage on L929 cells were analyzed.Resulst:The optimum extraction conditions of crude Aavps were as follows:Solid-liquid ratio of 1:150 g/mL,extraction time of 2 h,and extraction temperature of 70℃.Under these optimal conditions,the yield of crude Aavp was 8.57%.In the prevention test of H_(2)O_(2)oxidative damage on L929 cells,different doses of purified Aavp(20,50 and 100μg/mL)had a significant preventive effect on L929 cell oxidative damage(P<0.001)but did not show a dose effect.The cell survival rate of the middle dose group(50μg/mL)was the highest(71.94%±3.08%),which was slightly better than the positive control vitamin E group but not significant.In the repair test,different doses of purified Aavp(20,50 and 100μg/mL)had a significant repair effect on the oxidative damage of L929 cells(P<0.001)and showed a dose effect.In contrast,the cell survival rate of the high-dose group(100μg/mL)was the highest(90.93%±1.17%),slightly higher than that of the positive control vitamin E group(87.96%±3.05%).The results showed that purified Aavp showed an excellent preventive and repair effect on H_(2)O_(2)oxidative damage in L929 cells.Conclusion:Aavp has an ability to repair H_(2)O_(2)oxidative damage of L929 cells.

关 键 词：鲍鱼脏器 碱提多糖 响应面优化 氧化损伤 

分 类 号：TS201.1[轻工技术与工程—食品科学]