黑枸杞与桑葚花色苷纯化与结构分析  

Purification and Structural Analysis of Anthocyanins from Black Wolfberry and Mulberry

作　　者：刘恩芬 孙琳 陈子晔 王军[2] LIU Enfen;SUN Lin;CHEN Ziye;WANG Jun(Yunnan Zhongyan Reconstituted Tobacco Co.,Ltd.,Kunming 650001,China;College of Food Science and Nutritional Engineering,China Agricultural University,Beijing 100083,China)

机构地区：[1]云南中烟再造烟叶有限责任公司,云南昆明650001 [2]中国农业大学食品科学与营养工程学院,北京100083

出　　处：《食品工业科技》2024年第13期257-264,共8页Science and Technology of Food Industry

基　　金：中国农业大学校企科研基金(202305410610124)。

摘　　要：为研究富集、纯化黑枸杞和桑葚中花色苷的有效方法,以及鉴定花色苷结构。采用大孔树脂对黑枸杞和桑葚提取物进行纯化。比较了不同种类的大孔树脂对花色苷的吸附和解吸能力,筛选出LX-20SS大孔树脂为纯化黑枸杞花色苷的最佳树脂,纯化条件为:大孔树脂与黑枸杞花色苷粗粉的质量比为10:1、乙醇洗脱浓度为70%、洗脱流速为3 mL/min,纯化后花色苷含量为144.18±3.40 mg/g。选用DM-21大孔树脂为纯化桑葚花色苷的大孔树脂,纯化条件为:大孔树脂与桑葚花色苷粗粉的质量比为20:3、乙醇洗脱浓度为30%、洗脱流速为1 mL/min,纯化后花色苷含量为112.44±2.24 mg/g。高效液相色谱与质谱联用分析黑枸杞与桑葚中花色苷的结构显示,黑枸杞中主要含有的花色苷为矮牵牛色素-3-O-芸香糖(香豆酰基)-5-O-葡萄糖苷,占总花色苷比例的95%以上。桑葚中花色苷主要有两种,分别为矢车菊素-3-葡萄糖苷和矢车菊素-3-芸香糖苷,分别占总花色苷的63.58%和36.42%,为黑枸杞和桑葚的应用提供参考。In order to study the effective method of the enrichment and purification of anthocyanin in black wolfberry and mulberry,and identify the structure of anthocyanin,the black wolfberry and mulberry extracts were purified by macroporous resins.The adsorption and desorption abilities of different types of the resins for anthocyanins were compared.LX-20SS was optimized as the resin for the purification of anthocyanins from black wolfberry.The purification conditions were as follows:The mass ratio of resin to crude anthocyanin powder of black wolfberry was at 10:1,and the elution concentration of ethanol was 70%,and the flow rate was 3 mL/min.After the purification,the content of anthocyanin reached 144.18±3.40 mg/g.DM-21 was optimized as the resin for the purification of mulberry anthocyanin.The purification conditions were as follows:The mass ratio of resin to crude anthocyanin powder of mulberry was at 20:3,and the elution concentration of ethanol was 30%,and the flow rate was 1 mL/min.The content of anthocyanin reached 112.44±2.24 mg/g after purification.High performance liquid chromatography tandem mass spectrometry(HPLC-MS/MS)analysis for anthocyanin structures in black wolfberry and mulberry showed that the main anthocyanin in black wolfberry was petunidin-3-O-rutinose(p-coumaroyl)-5-O-glucoside,which accounted for more than 95%of the total anthocyanin.In addition,there are two main types of anthocyanins in mulberry,corresponding to cyanidin-3-glucoside and cyanidin-3-rutinoside,which accounted for 63.58%and 36.42%of the total anthocyanins respectively.The results provided references for the development of black wolfberry and mulberry.

关 键 词：黑枸杞 桑葚 花色苷 纯化 结构鉴定 

分 类 号：TS209[轻工技术与工程—食品科学]