植物乳杆菌LP21源纳米硒的分离纯化研究  

Study on isolation and purification of nano-selenium from Lactobacillus plantarum LP21

作　　者：王丽红[1] 王文斌 孔阳[1] WANG Li-hong;WANG Wen-bin;KONG Yang(School of Food Science and Engineering,Shaanxi University of Science&Technology,Xi′an 710021,China)

机构地区：[1]陕西科技大学食品科学与工程学院,陕西西安710021

出　　处：《陕西科技大学学报》2024年第4期63-69,93,共8页Journal of Shaanxi University of Science & Technology

基　　金：国家自然科学基金项目(32200974);陕西科技大学博士科研启动基金项目(2022BJ-30)。

摘　　要：植物乳杆菌LP21 (Lactobacillus plantarum LP21)合成的纳米硒易黏附于菌体表面,不易分离提取.本研究在保持细胞结构完整的前提下,对附着在菌体上的纳米硒进行分散处理.通过单因素实验探究超声波、有机溶剂及表面活性剂对纳米硒分散的影响,进而采用正交试验确定最佳分散条件,最后利用蔗糖密度梯度离心与微孔滤膜过滤的方法对纳米硒进行分离纯化.单因素结果表明:超声波、有机溶剂及表面活性剂均有分散作用,其中表面活性剂SDS效果最好;采用正交方法确定的最适分散条件为加入2.5%SDS、1.5%正己醇,超声处理时间25 min;分离纯化采用50%、60%、70%蔗糖密度梯度离心后用0.22μm的水性微孔滤膜过滤,可得到纯净的纳米硒.纳米硒颗粒分布均匀,平均粒径为165.88±35.35 nm.本研究为微生物来源纳米硒的分离制备提供参考依据.The nano-selenium synthesized by Lactobacillus plantarum LP21 is easy to adhere to the surface of the bacteria and is difficult to separate and extract.In this study,under the premise of maintaining the integrity of the cells structure,the nano-selenium attached to the bacteria was dispersed.The effects of ultrasound,organic solvents and surfactants on the dispersion of nano-selenium were investigated by single factor experiments.The optimal dispersion conditions were determined through orthogonal experiments.Finally,sucrose density gradient and microporous filter membrane filtration were used to separate and purify nano selenium.The results of single factor showed that ultrasonic,organic solvent and surfactant all had dispersion effect,among which surfactant SDS had the best effect.The optimum dispersion conditions were determined by orthogonal method to be the addition of 2.5%SDS and 1.5%n-hexanol,and the ultrasonic treatment time was 25 minutes.Separation and purification using 50%,60%,and 70%sucrose density gradient centrifugation followed by 0.22μm water-based microporous filter membrane filtration to obtain pure nano-selenium.The particles were evenly distributed and the average particle size was 165.88±35.35 nm.This study provides a reference for the separation and preparation of nano-selenium from microbial sources.

关 键 词：植物乳杆菌 纳米硒 分离纯化 

分 类 号：TS20[轻工技术与工程—食品科学] Q939.9[轻工技术与工程—食品科学与工程]