顶空固相微萃取法萃取茯砖茶挥发性成分条件的优化  

Optimization for Extraction of Volatile Compounds of Fuzhuan Tea by Headspace Solid-phase Microextraction

作　　者：赵静[1] 王滨 张静[1] 周天山[1] 余有本[1] 徐清山 ZHAO Jing;WANG Bin;ZHANG Jing;ZHOU Tianshan;YU Youben;XU Qingshan(College of Horticulture,Northwest A&F University,Yangling Shaanxi 712100,China)

机构地区：[1]西北农林科技大学园艺学院,陕西杨凌712100

出　　处：《西北农业学报》2024年第6期1102-1111,共10页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：国家自然科学基金青年项目(31902072);西北农林科技大学实验技术研究与实验室管理创新项目(SY20210226);陕西省农业厅茶产业专项(20200803000003)。

摘　　要：为更好地分析茯砖茶挥发性成分组成特征,应用气相色谱-质谱联用(GC-MS)检测平台,探讨萃取头涂层、茶水冲泡比例、萃取温度和萃取时间对萃取结果的影响。结果表明:DVB/CAR/PDMS(50/30μm)更适合萃取茯砖茶的挥发性成分;茯砖茶挥发性成分的最优萃取方法为:茶水比1:0、萃取时间70 min、萃取温度80℃。在此试验条件下,利用顶空固相微萃取从试验用的茯砖茶中检测到75种挥发性成分,包括碳氢类20种,醛类16种,酮类13种,醇类12种,甲氧基酚类9种,酯类4种,酚类1种。本研究明确了陕西代表性茯砖茶挥发性成分的萃取方法,为全面解析茯砖的香气品质提供可靠技术支撑。To better analyze the volatile compounds characteristics of Fuzhuan tea,the effects of different fiber coatings,tea to water ratio,extraction temperature and extraction time on extraction results were studied by gas chromatography-mass spectrometry(GC-MS).The results showed that DVB/CAR/PDMS(50/30μm)was the most suitable for extracting the volatile components of Fuzhuan tea;the optimal combination of experimental parameters was:tea to water ratio(g/mL)1:2,the extraction time 70 min,and the extraction temperature 80℃.As a result,75 volatile components were identified from Fuzhuan tea,which include 20 hydrocarbons,16 aldehydes,13 ketones,12 alcohols,9 methoxyphenols,4 esters,and 1 phenols.The volatile extraction method for Shaanxi Fuzhuan tea was clarified in this study,which provided a technical support for analyzing the aroma quality of Fuzhuan tea comprehensively.

关 键 词：茯砖茶 挥发性成分 固相微萃取 气相色谱-质谱联用 

分 类 号：O657.63[理学—分析化学] TS272.54[理学—化学]