瓜尔豆胶甘露寡糖对链脲佐菌素诱导糖尿病小鼠的降血糖作用  

Hypoglycemic Effect of Guar Gum Manno-Oligosaccharides on Streptozotocin-Induced Diabetic Mice

作　　者：程教擘 李延啸[2] 王楠楠[3] 闫巧娟[2] 江正强[1,4] CHENG Jiaobo;LI Yanxiao;WANG Nannan;YAN Qiaojuan;JIANG Zhengqiang(Key Laboratory of Food Bioengineering,China National Light Industry,College of Food Science and Nutritional Engineering,China Agricultural University,Beijing 100083,China;College of Engineering,China Agricultural University,Beijing 100083,China;College of Food Science and Engineering,Henan University of Technology,Zhengzhou 450001,China;Food Laboratory of Zhongyuan,Luohe 462333,China)

机构地区：[1]中国农业大学食品科学与营养工程学院,中国轻工业食品生物工程重点实验室,北京100083 [2]中国农业大学工学院,北京100083 [3]河南工业大学粮油食品学院,河南郑州450001 [4]中原食品实验室,河南漯河462333

出　　处：《食品科学》2024年第16期113-120,共8页Food Science

基　　金：“十四五”国家重点研发计划重点专项(2022YFD2101400)。

摘　　要：目的:研究瓜尔豆胶甘露寡糖(guar gum manno-oligosaccharides,GMOS)对链脲佐菌素诱导糖尿病小鼠的降血糖效果及作用机制。方法:采用GMOS干预糖尿病小鼠6周,监测小鼠体质量和胰岛素抵抗状态变化并测定血清中葡萄糖、胰岛素、高密度脂蛋白胆固醇(high density lipoprotein cholesterol,HDL-C)、低密度脂蛋白胆固醇(low density lipoprotein cholesterol,LDL-C)等糖脂代谢相关指标水平,观察肝脏组织形态学变化,同时检测肝脏中腺苷酸活化蛋白激酶(adenosine monophosphate-activated protein kinase,AMPk)/过氧化物酶增殖活化受体γ辅激活因子1α(peroxisome proliferator-activated receptorγcoactivator 1α,PGC-1α)信号通路相关蛋白的表达情况及抗氧化酶活性。结果:GMOS可以有效缓解糖尿病小鼠体质量减轻,明显改善其葡萄糖耐量(30.3%)和胰岛素敏感性(34.6%);体内葡萄糖、糖化血清蛋白和LDL-C含量较模型组小鼠分别降低38.6%、29.5%和27.3%,而胰岛素和HDL-C水平显著提高;有效减轻肝组织氧化损伤,激活肝脏AMPk/PGC-1α信号通路,提高肝脏内超氧化物歧化酶和过氧化氢酶活性。结论:GMOS具有降血糖效果,能够改善由糖尿病引发的糖脂代谢紊乱和氧化应激,具有作为抗糖尿病功能性食品配料的潜力。Objective:To study the hypoglycemic effect and mechanism of guar gum manno-oligosaccharides(GMOS)on streptozotocin-induced diabetic mice.Methods:GMOS was administered to diabetic mice for 6 weeks,and the changes in body mass and insulin resistance were monitored.Furthermore,indicators of glucose and lipid metabolism such as glucose(GLU),insulin(INS),high density lipoprotein cholesterol(HDL-C),and low density lipoprotein cholesterol(LDL-C)were determined.Hepatic histopathological changes were observed,and the expression of proteins involved in the adenosine monophosphate-activated protein kinase(AMPK)/peroxisome proliferator-activated receptorγcoactivator 1α(PGC-1α)signaling pathway and the activity of antioxidant enzymes in the liver were detected.Results:GMOS effectively relieved the body mass loss of diabetic mice,significantly alleviated glucose tolerance(30.3%)and enhanced insulin sensitivity(34.6%).Compared with the model group,GMOS decreased the levels of GLU,glycosylated serum protein(GSP)and LDL-C by 38.6%,29.5%,and 27.3%,respectively,and increased the levels of insulin and HDL-C significantly.Moreover,GMOS reduced oxidative damage,activated the AMPK/PGC-1αsignaling pathway,and improved the activities of superoxide dismutase(SOD)and catalase(CAT)in the liver.Conclusion:GMOS could reduce blood glucose and mitigate glucose and lipid metabolism disorder and oxidative stress caused by diabetes mellitus,indicating its potential as a functional food ingredient against diabetes.

关 键 词：瓜尔豆胶甘露寡糖 降血糖 糖脂代谢 信号通路 

分 类 号：TS201.4[轻工技术与工程—食品科学]