大环糊精的酶法制备及应用研究进展  

Research Progress on Enzymatic Preparation and Application of Large-Ring Cyclodextrin

作　　者：谢安宁 金征宇 李晓晓[1,2] 柏玉香 XIE Anning;JIN Zhengyu;LI Xiaoxiao;BAI Yuxiang(State Key Laboratory of Food Science and Resources,Jiangnan University,Wuxi 214122,China;School of Food Science and Technology,Jiangnan University,Wuxi 214122,China;Synergetic Innovation Center of Food Safety and Nutrition,Jiangnan University,Wuxi 214122,China;International Joint Laboratory on Food Safety,Jiangnan University,Wuxi 214122,China)

机构地区：[1]江南大学食品科学与资源挖掘全国重点实验室,江苏无锡214122 [2]江南大学食品学院,江苏无锡214122 [3]江南大学食品安全与营养协同创新中心,江苏无锡214122 [4]江南大学食品安全国际联合实验室,江苏无锡214122

出　　处：《食品科学》2024年第16期347-357,共11页Food Science

基　　金：江苏省自然科学优秀青年基金项目(BK20211581);江苏省自然科学基金项目(BK20221072);中央高校基本科研业务费专项资金资助项目(JUSRP221029;JUSRP422007)。

摘　　要：大环糊精(large ring cyclodextrin,LR-CD)是由9个至数百个吡喃葡萄糖单元组成的环状α-1,4-葡聚糖,主要通过环糊精葡萄糖基转移酶或4-α-糖基转移酶的环化作用使直链淀粉发生分子内转糖基作用得到。与常见α-、β-、γ-环糊精(cyclodextrin,CD)相比,LR-CD的空腔尺寸更大,表现出独有的结构和理化特性,这些性质使LR-CD在食品、医药、生物等领域展现出巨大的应用潜力。然而,LR-CD的制备成本高,产率较低,并且缺乏有效的分离纯化方法,因此目前还未实现工业化生产与应用。本文分别从优化底物、设计基于模板诱导的新反应路径、酶分子改造及产物的分离纯化等方面综述酶法制备LR-CD的研究近况与挑战,为LR-CD的工业化生产和应用提供参考。Large-ring cyclodextrin(LR-CD)is a cyclicα-1,4-glucan consisting of nine to several hundred glucopyranose units.LR-CD is mainly prepared by the cyclization activity of cyclodextrin glucosyltransferase or 4-α-glucanotransferase that catalyzes intramolecular transglycosylation reaction on amylose.Compared with commonα-,β-,andγ-CD,LR-CD has a larger cavity size and exhibits unique structural and physicochemical properties,showing potential applications in several fields like food,medicine and biology.However,LR-CD has not been industrially produced or applied due to the high cost and low productivity as well as lack of effective separation and purification methods.In this article,recent progress and challenges on the enzymatic preparation of LR-CD are reviewed from the aspects of optimizing reaction substrates,designing new reaction pathways based on template induction,enzyme modification,and separation and purification of products.We hope this article could provide a reference for the industrial production and application of LR-CD.

关 键 词：大环糊精 环糊精葡萄糖基转移酶 4-α-糖基转移酶 淀粉 模板诱导 分子改造 

分 类 号：TS236.9[轻工技术与工程—粮食、油脂及植物蛋白工程]