高温大曲中1株产四甲基吡嗪细菌的鉴定及生物特性分析  

Identification and Biological Characteristics Analysis of a Tetramethylpyrazine-producing Bacterium from High-temperature Daqu

作　　者：赖宝玲 黄娜婷 黄远龙 张守财 黄祖新[1] 李善仁 LAI Bao-ling;HUANG Na-ting;HUANG Yuan-long;ZHANG Shou-cai;HUANG Zu-xin;LI Shan-ren(College of Life Sciences,Fujian Normal University,Fuzhou,Fujian 350117,China;Huanghuashan Liquor-making Co.Ltd.,Jian’ou,Fujian 353100,China)

机构地区：[1]福建师范大学生命科学学院,福建福州350117 [2]福建省建瓯黄华山酿酒有限公司,福建建瓯353100

出　　处：《福建农业科技》2024年第5期10-15,共6页Fujian Agricultural Science and Technology

基　　金：福建省科技计划区域发展项目(2021N3011);福建师范大学大学生创新创业训练计划(cxxl-2024283)。

摘　　要：四甲基吡嗪是白酒中重要的风味物质,赋予了白酒的健康功效。采用稀释涂布平板法从贵州高温大曲中分离产四甲基吡嗪的细菌,通过菌落形态、显微镜观察和分子生物学方法对其进行鉴定,并对其产酶特性、生长特性及耐受特性进行研究。结果表明:从贵州高温大曲中筛选得到1株产四甲基吡嗪的菌株FSDQ02,初步鉴定为解淀粉芽孢杆菌Bacillus amyloliquefaciens,该菌能分泌蛋白酶、淀粉酶和纤维素酶;能在30~40℃和pH5~9范围内生长良好;对6%的乙醇和14%的NaCl具有耐受性。该研究结果分离得到的解淀粉芽孢杆菌FSDQ02在强化高温大曲和白酒生产中具有较大的应用潜力。Tetramethylpyrazine is an important flavor compound in liquor,which endows liquor with health efficacy.The bacteria that could produce tetramethylpyrazine were isolated from the high-temperature Daqu in Guizhou by using the dilution spread plate method,and were identified by using the methods such as colonial morphology,microscopic observation and molecular biology.Then,their enzyme production characteristics,growth characteristics and tolerance characteristics were studied.The results showed that one strain FSDQ02 producing tetramethylpyrazine was screened from the high-temperature Daqu in Guizhou,which was preliminarily identified as Bacillus amyloliquefaciens.The strain FSDQ02 could secrete protease,amylase and cellulase,and grow well in the range of 30-40℃and pH 5-9,with the tolerance to 6%ethanol and 14%NaCl.Bacillus amyloliquefaciens FSDQ02 isolated from the results of this study had great great application potential in strengthening the production of high-temperature Daqu and liquor.

关 键 词：高温大曲 四甲基吡嗪 生物学特性 解淀粉芽孢杆菌 

分 类 号：TS261.4[轻工技术与工程—发酵工程]