黑曲霉全细胞催化甘蔗糖蜜生产低聚果糖研究  

Study on the Production of Oligofructose from Sugarcane Molasses Catalyzed by Whole Cell of Aspergillus niger

作　　者：雷光鸿 宁方尧[1] 李元 陈亮[2] 黄凯[1] 王达 漆全 刘葵研 程昊[3] LEI Guanghong;NING Fangyao;LI Yuan;CHEN Liang;HUANG Kai;WANG Da;QI Quan;LIU Kuiyan;CHENG Hao(Guangxi Vocational&Technical Institute of Industry,Nanning,Guangxi 530001;Guangxi University,Nanning,Guangxi 530004;Guangxi Key Laboratory of Green Processing of Sugar Resources/Guangxi university of science and Technology,Liuzhou,Guangxi 545006)

机构地区：[1]广西工业职业技术学院,广西南宁530001 [2]广西大学,广西南宁530004 [3]广西糖资源绿色加工重点实验室/广西科技大学,广西柳州545006

出　　处：《甘蔗糖业》2024年第3期61-66,共6页Sugarcane and Canesugar

基　　金：广西工业职业技术学院科学研究项目(KYHT2024040);广西高校中青年教师科研基础能力提升项目(桂工业院科研2020001KY025);广西糖资源绿色加工重点实验室开放课题(GXTZ201905、GXTZY201904)。

摘　　要：为了探明黑曲霉全细胞催化产低聚果糖(FOS)的变化规律,本研究采用单因素实验,HPLC检测FOS含量。结果表明:全细胞催化产FOS单因素优化条件为柠檬酸缓冲液、pH值6.0、湿菌丝体种龄48 h、全细胞催化剂浓度120 g/L、底物蔗糖浓度400 g/L、转化温度50℃。在此生产工艺条件下,低聚果糖的产量达到了201.23 g/L,超过目前报道糖蜜生产低聚果糖160 g/L,达到了较高的生产水平。In order to explore the change rule of oligofructose(FOS)production by Aspergillus niger,single factor experiment was used to detect the content of FOS by HPLC.The results showed that the optimal conditions for FOS production were citric acid buffer,pH 6.0,wet mycelium age 48 h,whole cell catalyst concentration 120 g/L,substrate sucrose concentration 400 g/L,and conversion temperature of 50℃.Under these conditions,the yield of FOS reached 201.23 g/L,which was higher than the reported production of 160 g/L oligofructose from molasses.

关 键 词：甘蔗糖蜜 低聚果糖 黑曲霉 生物转化 

分 类 号：TS249[轻工技术与工程—制糖工程]