伊力特浓香型大曲中优良霉菌的筛选鉴定及其麸曲制曲工艺研究  

Screening and identification of superior mold in Yilite strong-flavor Daqu and research on Fuqu-making technology

作　　者：张家辉 海超 陈国刚[1] ZHANG Jiahui;HAI Chao;CHEN Guogang(School of Food Science,Shihezi University,Shihezi 832000,China;Xingjiang Yilite Industry Co.,Ltd.,Cocodala 835213,China)

机构地区：[1]石河子大学食品学院,新疆石河子832000 [2]新疆伊力特实业股份有限公司,新疆可克达拉835213

出　　处：《中国酿造》2024年第8期93-99,共7页China Brewing

基　　金：兵团科技计划项目(2021AB005,2023AB003)。

摘　　要：该研究采用传统培养分离法从伊力特浓香型大曲中分离筛选高产糖化酶和液化酶活力的霉菌,并通过形态学观察及分子生物学技术对其进行菌种鉴定。利用筛选菌株制作麸曲,并以糖化酶及液化酶活力为评价指标,采用单因素试验及响应面试验对其制曲条件进行优化。结果表明,筛选得到一株产糖化酶、液化酶性能优良的霉菌,编号为JW-3,经鉴定其为伞枝横梗霉(Lichtheimia corymbifera)。使用伞枝横梗霉JW-3制作纯菌种麸曲的最佳制曲条件为接种量10%,培养温度30℃,培养时间91 h,水分含量55%。在此优化条件下,霉菌纯菌种麸曲的糖化酶活力为409.67 U/g,液化酶活力为1597.00 U/g,在白酒酿造过程中具有一定的应用潜力。The molds with high saccharifying enzyme and liquefying enzyme activity were isolated and screened by traditional cultivation and separation methods from Yilite strong-flavor(Nongxiangxing)Daqu,and identified through morphological observation and molecular biology techniques.Fuqu was made using screened strains,and the Fuqu-making conditions were optimized by single factor experiments and response surface experiments using saccharifying enzyme and liquefying enzyme activity as evaluation indicators.The results showed that a strain of mold with superior performance in producing saccharifying enzyme and liquefying enzymes was screened,numbered JW-3,and the strain JW-3 was identified as Lichtheimia corymbifera.The optimal Fuqu-making conditions for the production of pure strain Fuqu with L.corymbifera JW-3 were obtained as follows:inoculum 10%,culture temperature 30℃,time 91 h,and water content 55%.Under these optimal conditions,the saccharifying enzyme activity of pure mold Fuqu was 409.67 U/g,and the liquefying enzyme activity was 1597.00 U/g,which had certain application potential in Baijiu brewing.

关 键 词：浓香型大曲 霉菌 筛选 鉴定 麸曲 响应面法 

分 类 号：TS262.3[轻工技术与工程—发酵工程]