β-葡萄糖苷酶产生菌的筛选鉴定及其粗酶液酶学性质研究  

Screening and identification ofβ-glucosidase-producing strain and enzymatic properties of crude enzyme

作　　者：覃宝山 何海燕 李燕婷 付跃 覃拥灵 QIN Baoshan;HE Haiyan;LI Yanting;FU Yue;QIN Yongling(School of Chemistry and Bioengineering,Hechi University,Hechi 546300,China;Guangxi Colleges Universities Key Laboratoryof Exploitation and Utilization of Microbial and Botanical Resources,School of Chemistry and Bioengineering,Hechi University,Hechi 546300,China;Guangxi Key Laboratory of Sericulture Ecology and Applied Intelligent Technology,School of Chemistry and Bioengineering,Hechi University,Hechi 546300,China;Guangxi Collaborative Innovation Center of Modern Sericulture and Silk,School of Chemistry and Bioengineering,Hechi University,Hechi 546300,China;Applied Research Center forAgricultural Biotechnology,School of Chemistry and Bioengineering,Hechi University,Hechi 546300,China)

机构地区：[1]河池学院化学与生物工程学院,广西河池546300 [2]河池学院化学与生物工程学院微生物及植物资源开发利用广西高校重点实验室,广西河池546300 [3]河池学院化学与生物工程学院广西蚕桑生态学与智能化技术应用重点实验室,广西河池546300 [4]河池学院化学与生物工程学院广西现代蚕桑丝绸协同创新中心,广西河池546300 [5]河池学院化学与生物工程学院农业生物技术应用研究中心,广西河池546300

出　　处：《中国酿造》2024年第8期128-132,共5页China Brewing

基　　金：广西自然科学基金(2020GXNSFAA297218);河池学院重点课题(2021XJZD006)。

摘　　要：该研究从原始森林腐木的土壤中取样,筛选产β-葡萄糖苷酶菌株,对其产β-葡萄糖苷酶能力进行测定,并对高产菌株进行形态特征、分子生物学鉴定及酶学性质研究。结果表明,筛选得到13株产β-葡萄糖苷酶菌株,复筛得到1株高产β-葡萄糖苷酶的菌株PX14A,其β-葡萄糖苷酶酶活为4.9 U/mL。菌株PX14A被鉴定为枯草芽孢杆菌(Bacillus subtilis)。酶学性质研究结果表明,菌株PX14A粗酶液的β-葡萄糖苷酶在65℃以下稳定,最适反应温度为60℃,在pH值4.0~7.5较稳定,最适反应pH值为6.5,浓度为1 mmol/L的Mg^(2+)和Fe^(2+)对酶活性有较好的激活作用。结果显示该菌株具有良好应用前景。β-glucosidase-producing strains were selected from the soil of decayed trees in primitive forests,and theβ-glucosidase production ability was measured.The morphological characteristics,molecular biological identification and enzymatic properties of the high-yield strains were studied.The results showed that 13β-glucosidase-producing strains were screened,a high-yield glucosidase strain PX14A was obtained through re-screening,and theβ-glucosidase activity was 4.9 U/ml.The strain PX14A was identified as Bacillus subtilis.The results of enzymatic properties research showed that theβ-glucosidase of the strain PX14A crude solution was stable below 65℃,the optimal reaction temperature was 60℃,the enzyme was stable at pH 4.0-7.5,and the optimal reaction pH value was 6.5.Mg2+and Fe2+with a concentration of 1 mmol/L had a good activating effect on enzyme activity.The results indicated that the strain PX14A had good application prospects.

关 键 词：Β-葡萄糖苷酶 筛选 鉴定 酶学性质 

分 类 号：TQ925[轻工技术与工程—发酵工程] TS201.3