玉米黄素减轻脂多糖诱导的3T3-L1脂肪细胞慢性炎症  

Zeaxanthin Alleviates Chronic Inflammation of 3T3-L1 Adipocytes Induced by Lipopolysaccharide

作　　者：赵有伟 吴熙 朱晨 刘美宏[1] 郑明珠[1] 蔡丹[1] 刘回民[1] 刘景圣[1] Zhao Youwei;Wu Xi;Zhu Chen;Liu Meihong;Zheng Mingzhu;Cai Dan;Liu Huimin;Liu Jingsheng(College of Food Science and Engineering,Jilin Agricultural University,National Engineering Research Centre for Deep Processing of Wheat and Corn,Changchun 130118)

机构地区：[1]吉林农业大学食品科学与工程学院小麦和玉米深加工国家工程研究中心,长春130118

出　　处：《中国食品学报》2024年第7期47-59,共13页Journal of Chinese Institute Of Food Science and Technology

基　　金：吉林省自然科学基金项目(20200201148JC);吉林省中青年科技创新创业卓越人才(团队)项目(创新类)(20210509026RQ)。

摘　　要：目的:探究一种天然类胡萝卜素——玉米黄素(ZEA),基于NF-κB信号通路减轻脂多糖(LPS)诱导的3T3-L1脂肪细胞慢性炎症并发挥抗炎的作用机制。方法:以LPS刺激成熟的3T3-L1脂肪细胞建立炎症模型,用不同浓度的ZEA(0,5,10,15μmol/L)进行干预,检测脂肪细胞炎症相关因子的含量,并通过实时荧光定量PCR(qPCR)检测其mRNA表达水平,用蛋白免疫印迹法检测信号通路因子的蛋白表达水平。结果:与LPS组相比,ZEA干预显著抑制炎症细胞模型中促炎因子IL-1β、IL-6、TNF-α和MCP-1分泌(P<0.001),同时促进抗炎因子IL-10和ADPN产生(P<0.001)。此外,qPCR结果显示ZEA能有效抑制促炎因子,同时恢复抗炎因子的mRNA表达水平(P<0.001)。蛋白免疫印迹结果表明,ZEA能明显降低IκB激酶催化亚基IKKα和IKKβ的蛋白和磷酸化表达(P<0.001),增强抑制蛋白IκBα与核转录因子NF-κB p65的结合,最终抑制NF-κB p65进行核内转录。结论:ZEA可通过抑制NF-κB信号通路激活及调控炎性因子的分泌,改善肥胖引起的慢性炎症。Objective:To explore the anti-inflammatory mechanism of zeaxanthin(ZEA,a natural carotenoid)based on the NF-κB signaling pathway in lipopolysaccharide-induced(LPS-induced)3T3-L1 adipocytes chronic inflammatory.Methods:The inflammatory models were established by using LPS to stimulate mature 3T3-L1 adipocytes and were intervened with different concentrations of ZEA(0,5,10,15μmol/L).The content of inflammatory-related cytokines was measured,and their mRNA expression levels were detected by Quantitative Real-time PCR(qPCR).In addition,the mRNA and protein expression levels of signaling pathway cytokines were detected by Western blot.Results:Compared with the normal group,ZEA intervention significantly down-regulated the secretion of pro-inflammatory cytokines IL-1β,IL-6,TNF-αand MCP-1(P<0.001)while up-regulated the content of anti-inflammatory cytokines IL-10 and ADPN(P<0.001)in the inflammatory models.Moreover,qPCR indicated that ZEA inhibited the mRNA expression of pro-inflammatory cytokines(P<0.001)while recovering the mRNA expression of anti-inflammatory cytokines(P<0.001).Furthermore,Western blot analysis suggested that ZEA notably decreased the protein and phosphorylation expression of catalytic subunits of IκB kinases(IKKαand IKKβ)(P<0.001),then increased the combination of inhibitory protein IκBαand nuclear transcription factor NF-κB p65,and eventually inhibited the transcription of NF-κB p65 in the nucleus.Conclusions:Zeaxanthin effectively alleviates obesity-caused chronic inflammation by inhibiting NF-κB signaling pathway activation and regulating inflammatory cytokines secretion.

关 键 词：玉米黄素 脂多糖 慢性炎症 NF-ΚB信号通路 抗炎作用 

分 类 号：TS201.4[轻工技术与工程—食品科学]