响应面法优化“水城春”绿茶茶多酚的冲泡条件及其抗氧化活性  

The Optimization of Brewing Conditions and Antioxidant Activity of Polyphenols from Shuichengchun Green Tea Using Response Surface Method

作　　者：李金辉 张泽敏 孙蓉 罗帆 潘宇荷 翁贵英 Li Jinhui;Zhang Zemin;Sun Rong;Luo Fan;Pan Yuhe;Weng Guiying(School of Chemistry and Materials Engineering,Liupanshui Normal University,Liupanshui 553004,China;School of Biological Science and Technology,Liupanshui Normal University,Liupanshui 553004,China)

机构地区：[1]六盘水师范学院化学与材料工程学院,贵州六盘水553004 [2]六盘水师范学院生物科学与技术学院,贵州六盘水553004

出　　处：《六盘水师范学院学报》2024年第3期11-18,共8页Journal of Liupanshui Normal University

基　　金：贵州省教育厅高校科技创新团队项目“贵州省高等学校精细化学品清洁合成创新团队”(黔教技〔2023〕088号);六盘水师范学院大学生科研训练计划项目“响应面法优化水城春茶多酚的冲泡工艺及抗氧化活性研究”(2022DK027);六盘水师范学院科技创新团队项目“天然产物综合利用创新团队”(LPSSYKJTD201904)。

摘　　要：以“水城春”绿茶为原料,采用响应面法优化“水城春”绿茶茶多酚的冲泡条件,并测定其对DPPH·和ABTS+·的清除率,评价最佳冲泡条件下“水城春”绿茶茶多酚的抗氧化活性。结果表明:“水城春”绿茶茶多酚最佳冲泡条件为冲泡3次,100 mL水中茶添加量0.5542 g、冲泡时间12 min、冲泡温度92℃;在此条件下,茶多酚溶出率为14.52%,与预测值14.60%接近,茶多酚提取率为73.33%。当“水城春”绿茶茶多酚浓度为16.18μg/mL时,其对DPPH·和ABTS+·的清除率分别为90.23%、91.80%,清除能力均优于同浓度Vc溶液。由此可见,“水城春”绿茶具有较好的抗氧化活性。To evaluate the antioxidant activity of tea polyphenols in Shuichengchun green tea under optimal brewing conditions.Using commercially available Shuichengchun green tea as raw material,the response surface methodology was used to optimize brewing conditions of polyphenols,and the scavenging rates of DPPH·and ABTS+·were measured.The results showed that the optimal brewing conditions for Shuichengchun green tea were 3 times,with a tea addition of 0.5542 g in 100 mL of water,a brewing time of 12 minutes,and a brewing temperature of 92℃.Under the conditions,the dissolution rate of tea polyphenols was 14.52%,which was close to the predicted value of 14.60%.The extraction rate of tea polyphenols reached 73.33%.The scavenging rate of DPPH·and ABTS+·are 90.23%and 91.80%when the concentration of polyphenols is 16.18μg/mL.It can be inferred that the polyphenols in Shuichengchun green tea have better scavenging abilities for DPPH·and ABTS+·than Vc solutions of the same concentration.Therefore,Shuichengchun green tea has good antioxidant activity.

关 键 词：“水城春”绿茶 茶多酚 冲泡条件 抗氧化活性 

分 类 号：S571.1[农业科学—茶叶生产加工]