Carbonic anhydrase 2 mediates anti-obesity effects of black tea as thermogenic activator  

作　　者：Peng Ma Jie Xiao Biyu Hou Ping He Xinyu Yang Yisa Wang Zijing Wang Tianshu Xu Xiuying Yang Xuan Zhu Shasha Xiang Song Li Guanhua Du Jian Ying Guifen Qiang 

机构地区：[1]State Key Laboratory of Bioactive Substance and Function of Natural Medicines,Institute of Materia Medica,Chinese Academy of Medical Sciences and Peking Union Medical College and Beijing Key Laboratory of Drug Target and Screening Research,Beijing 100050,China [2]COFCO Nutrition and Health Research Institute,Beijing 102209,China [3]Department of Pharmacy,The Eighth Affiliated Hospital,Sun Yat-sen University,Shenzhen 518000,China [4]Faculty of Biology,Medicine and Health Sciences,The University of Manchester,Manchester M139PL,United Kingdom [5]Changchun University of Chinese Medicine,Changchun 130117,China [6]School of Food Science and Biotechnology,Zhejiang Gongshang University,Hangzhou 310018,China [7]China Tea Technology(Beijing)Co.,Ltd.,Beijing 102209,China [8]Beijing Key Laboratory of Nutrition,Health and Food Safety,Beijing 102209,China

出　　处：《Food Science and Human Wellness》2024年第5期2917-2936,共20页食品科学与人类健康（英文）

基　　金：funded by National Natural Science Foundation of China(NSFC 82070877);CAMS Innovation Fund for Medical Sciences(CIFMS)(2022-I2M-JB-010,2021-I2M-1-005);The National High Technology Research and Development Program of China(2017YFE0112900).

摘　　要：Obesity is a metabolic disorder due to over-accumulation of adipose tissue and ultimately becomes a“disease”.Brown adipose tissue(BAT)thermogenesis and white adipose tissue(WAT)browning emerge as a potential strategy of anti-obesity by dissipating energy as heat.However,drugs based on adipose tissue thermogenesis have not been successfully approved yet.In current study,we found that black tea extract(BTE)obtained by patentauthorized manufacturing process prevented body weight gain as novel thermogenic activator with reduction of adiposity,improvement of adipose distribution,and glucose metabolism improvement in diet-induced obesity mice.Mechanismly,anti-obesity effect of BTE depends on promoting BAT thermogenesis and WAT browning with upregulation of uncoupling protein 1(UCP1),especially visceral adipose tissue(VAT)with browning resistance.Specifically,utilizing in silico approach of network pharmacology and molecular docking,we identified carbonic anhydrase 2(CA2)in nitrogen metabolism as anti-obesity target of BTE and further elucidated that protein kinase B(AKT)signaling pathway linked CA2 and UCP1.Meanwhile gut microbiota regulation may prompt the CA2-dependent thermogenesis activation.Our findings demonstrated anti-obesity effect of BTE as thermogenic activator through CA2-mediated BAT thermogenesis and WAT browning via CA2-AKT-UCP1 signaling pathway,which could be developed as promising anti-obesity agent with good safety and efficacy.

关 键 词：OBESITY Black tea THERMOGENESIS BROWNING Adipose tissue 

分 类 号：TS201.2[轻工技术与工程—食品科学] TS272.52[轻工技术与工程—食品科学与工程] R589.2[农业科学—茶叶生产加工]