甜菜汁中耐高糖乳酸菌的筛选及其发酵生产胞外多糖的抗氧化活性研究  

Screening of high sugar tolerant lactic acid bacteria and study on the antioxidant activity of extracellular polysaccharides produced by fermentation in beet juice

作　　者：张菡 任高宇 甘好 陈海军 李秋晓 赵抒娜 申光辉[2] ZHANG Han;REN Gao-Yu;GAN Hao;CHEN Hai-Jun;LI Qiu-Xiao;ZHAO Shu-Na;SHEN Guang-Hui(National Postdoctoral Programme/Xinjiang Engineering Research Center of Beet Sugar,COFCO Sugar Holding Co.,Ltd.,Changji 831100,China;College of Food Science/Key Laboratory of Agro-products Processing and Nutritional Health(Co-construction by Ministry and Province),Ministry of Agriculture and Rural Affairs,Sichuan Agricultural University,Ya’an 625014,China;School of Food and Health/Key Laboratory of Geriatric Nutrition and Health,Ministry of Education,Beijing Technology and Business University,Beijing 100048,China)

机构地区：[1]中粮糖业控股股份有限公司,国家博士后科研工作站/新疆甜菜制糖工程研究中心,昌吉831100 [2]四川农业大学食品学院/农业农村部农产品加工与营养健康重点实验室(部省共建),雅安625014 [3]北京工商大学食品与健康学院/老年营养与健康教育部重点实验室,北京100048

出　　处：《食品安全质量检测学报》2024年第16期262-270,共9页Journal of Food Safety and Quality

基　　金：新疆昌吉州科技计划项目(2022X01);新疆昌吉州科技支撑产业高质量发展专项(2023X05);中粮糖业控股股份有限公司-四川农业大学校企合作项目(060-2222319002)。

摘　　要：目的挖掘甜菜浸出汁中耐高糖且高产胞外多糖乳酸菌,评估其生物转化甜菜糖蜜开发功能胞外多糖的潜力。方法以胞外多糖产量和耐高糖生长能力对分离自甜菜浸出汁的乳酸菌菌株进行双重筛选,通过形态特征结合16S rRNA测序对1株较强应用潜力的菌株COFL4进行鉴定,采用单因素结合Box-Behnken响应面法对COFL4利用甜菜糖蜜合成胞外多糖的摇瓶发酵条件进行优化,并对获得的胞外多糖体外抗氧化活性进行评价。结果菌株COFL4被鉴定为植物乳植杆菌(Lactiplantibacillus plantarum),在蔗糖质量浓度450 g/L培养基中生长良好,其利用甜菜糖蜜发酵胞外多糖的最佳条件为:甜菜糖蜜添加量30%,发酵温度31℃、摇床转速32 r/min、装液量180 mL/250 mL、初始pH为7.0、发酵时间36 h,此条件下胞外多糖产量为12.73 g/L,较优化前提高了79%。体外抗氧化活性表明,COFL4发酵获得的胞外多糖具有良好的抗氧化活性。结论植物乳植杆菌COFL4具有生产抗氧化活性胞外多糖的潜在前景。Objective To explore the high glucose-tolerant and high exopolysaccharides-producing lactic acid bacteria isolated from sugar beet juice and to assess their potential for bioconversion of sugar in the molasses to functional exopolysaccharides.Methods Lactic acid bacteria strains isolated from beet juice were subjected to a double screening process to assess their potential for exopolysaccharides yield and high sucrose tolerance.Strain COFL4 was identified as a promising candidate for further investigation based on its morphological characteristics and 16S rRNA analysis.The shaking flask fermentation conditions for the synthesis of exopolysaccharides from beet molasses by COFL4 were optimized using a one-way combination of Box-Behnken response surface methodology.The antioxidant activity in vitro of exopolysaccharides from COFL4 was evaluated.Results The strain COFL4 was identified as Lactiplantibacillus plantarum,which grew well in sucrose mass concentration of 450 g/L,and its optimal conditions for exopolysaccharides yield from sugar beet molasses were:30%addition of sugar beet molasses,fermentation temperature of 31°C,shaker speed of 32 r/min,loading volume of 180 mL/250 mL,initial pH of 7.0,and fermentation time of 36 h.The exopolysaccharides yield under these conditions was 12.73 g/L,which was 79%higher than that before optimization.The in vitro antioxidant activity showed that exopolysaccharides from COFL4had good antioxidant activity.Conclusion Lactiplantibacillus plantarum COFL4 represents a promising strain for the conversion of sugar in beet molasses to exopolysaccharides with good antioxidant activity.

关 键 词：甜菜糖蜜 植物乳植杆菌 胞外多糖 发酵条件优化 抗氧化活性 

分 类 号：TS201.3[轻工技术与工程—食品科学]