燕麦β-葡聚糖益生元组合配方对肠道健康的改善作用  

IMPROVEMENTS OF GUT HEALTH BY OATβ-GLUCAN BASED PREBIOTIC BLENDING FORMULAS IN ADULTS WITH DEFECATION DIFFICULTY

作　　者：李蔼琪 金磊 王欣[3] 徐海 刘烈刚[2] 孙桂菊[1] LI Ai-qi;JIN Lei;WANG Xin;XU Hai;LIU Lie-gang;SUN Gui-ju(Laboratory of Environmental Medicine and Engineering of Ministry of Education,and Department of Nutrition and Food Hygiene,School of Public Health Southeast University,Nanjing 210009;Department of Nutrition and Food Hygiene,Hubei Key Laboratory of Food Nutrition and Safety,School of Public Health,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030;Institute of Microbiology,Zhejiang Academy of Agricultural Sciences,Hangzhou 310022,China)

机构地区：[1]东南大学公共卫生学院教育部环境医学工程重点实验室/营养与食品卫生学系,南京210009 [2]华中科技大学同济医学院公共卫生学院,武汉430030 [3]浙江省农业科学院微生物研究所,杭州310022

出　　处：《营养学报》2024年第3期228-234,共7页Acta Nutrimenta Sinica

摘　　要：目的探究以燕麦β-葡聚糖为基础的不同益生元组合配方对肠道健康的影响。方法采用人体粪便体外发酵方法,对两个以燕麦β-葡聚糖为基础的益生元组合配方进行24 h和48 h发酵,通过qPCR和16S rDNA的方法探究配方对肠道菌群的影响。之后采用双中心平行双盲随机对照人群试验,选取192名排便困难的18~65岁的健康成人,随机分为3组,分别干预28 d,试验期间收集受试者的粪便,并记录受试者排便情况。采用线性混合效应模型、t检验和协方差分析探讨益生元组合配方对肠道菌群、排便状况和肠道健康状况的影响。结果体外试验和人群试验显示,相比于对照组,以燕麦β-葡聚糖为基础的益生元组合配方可以促进肠道有益菌普拉梭菌(Faecalibacterium prausnitzii)、黏蛋白艾克曼氏菌(Akkermansia muciniphila)、霍尔德曼氏菌属(Holdemania)和布劳特氏菌属(Blautia)的增殖(P<0.05)。经人群试验验证,相比于普通膳食纤维,燕麦β-葡聚糖为基础的两个益生元组合能改善排便,以及腹胀、消化不良等肠道健康问题(P<0.05)。结论以燕麦β-葡聚糖为基础的益生元组合配方,可促进肠道有益菌增殖,改善肠道健康。Objective To investigate the effects of oatβ-glucan based prebiotic blending formulas on gut health.Methods The 24 h and 48 h in vitro fermentations on two oatβ-glucan based prebiotic blending formulas using human feces were conducted.The effects of two formulas on gut bacteria were explored by qPCR and 16S rDNA approaches.Subsequently,a two-center parallel double-blind randomized controlled human trial was conducted in 192 adults with defecation difficulty.The participants were randomly assigned to one of three groups with a 28-day intervention.During the intervention,human fecal samples were collected and defecation feelings were investigated.The data were analyzed using generalized mixed linear model,t-test and ANCOVA.Results The results of both in vitro test and human trial found that,compared to control group,oatβ-glucan based prebiotic blending formulas could promote the growth of Faecalibacterium prausnitzii,Akkermansia muciniphila,Holdemania and Blautia(P<0.05).Oatβ-glucan based prebiotic blending formulas also improved the defecation difficulty and digestive feeling(P<0.05).Conclusion Oatβ-glucan based prebiotic blending formulas can promote the growth of gut beneficial bacteria,and improve gut health in adults with defecation difficulty.

关 键 词：燕麦Β-葡聚糖 益生元 体外发酵 人群试验 肠道菌群 排便 

分 类 号：R151.42[医药卫生—营养与食品卫生学]